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Transgenic mice expressing human copper-zinc superoxide dismutase exhibit attenuated apparent diffusion coefficient reduction during reperfusion following focal cerebral ischemia


, : Transgenic mice expressing human copper-zinc superoxide dismutase exhibit attenuated apparent diffusion coefficient reduction during reperfusion following focal cerebral ischemia. Brain Research 947(1): 1-8, 23 August

Since ADC reduction reflects intracellular edema which is an early indicator of ischemic cellular metabolic stress, we hypothesized that a decrease in ADC as determined by diffusion weighted MR imaging could be attenuated by SOD expression in transgenic mice during reperfusion following focal cerebral ischemia. Diffusion weighted imaging (DWI) was performed to evaluate apparent diffusion coefficient (ADC) reduction by constructing ADC maps with a color scale to localize ADC change in transgenic (Tg) mice expressing human CuZn superoxide dismutase (SOD) and wild type (Wt) mice during 1 h middle cerebral artery occlusion (MCAO) and 1 h reperfusion. Heat shock protein (hsp) 70-kDa mRNA analysis was evaluated as a marker of sublethal cell stress by in situ hybridization after 4 h reperfusion for comparison with Nissl staining of adjacent sections to assess infarction. Sequential ADC maps were prepared in Tg mice with sufficient temporal and spatial resolution to permit comparison with Wt mice. Tg mice showed substantial recovery of the ADC lesion after reperfusion, while Wt mice showed no recovery. There was no difference between Tg and Wt mice in the size or distribution of the ADC lesion during ischemia. The area with strong expression of hsp70 mRNA in the ischemic hemisphere was substantially larger in the Tg mice. Nissl staining showed less damage of brain tissue in Tg mice than Wt mice especially in the cortex after 4 h reperfusion following 1 h MCAO. Results demonstrate that antioxidant effects of human CuZn-SOD reduce cellular edema due to oxidative stress during reperfusion but not during ischemia after 1 h MCAO. Hsp70 could be one of the proteins that mediates protection by SOD against oxidative stress.

US$19.90

PMID: 12144846

DOI: 10.1016/s0006-8993(02)02899-8


Other references

Crawford, A.B., 1978: State and federal responses to the 1977 drought. By 1977, nearly four-fifths of the counties in the United States had declared drought areas for purposes of federal relief. Responses by several different governmental levels are described. In the west, a number of multilateral state organizations...

Sosa Bourdouil, C., 1949: Sur le chimisme des graines de Datura tetraploides. A study of colchicine-produced tetraploid and normal diploid seeds of Datura showed differences. The tetraploid seeds weighed more and contained more lipids. There was very little difference in the nucleic acids. The alkaloids were found in very s...

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Wunderlich, P., 1963: Peritonwillar Abscesses In Childhood And Their Differential Diagnosis. Kinderarztliche Praxis 31: 207-211

Bertagnolli, B.L.; Daly, S.; Sinclair, J.B., 1998: Antimycotic compounds from the plant pathogen Rhizoctonia solani and its antagonist Trichoderma harzianum. The soilborne rhizosphere-competent fungal biocontrol agent Trichoderma harzianum isolate Th008 secreted trichodermin (MW = 292) and a small peptide (MW = 876) in culture. These compounds were antagonistic in culture to the mycelial growth of the...

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Campbell, Susan, 1980: Cooks tools, the complete manual of kitchen implements and how to use them. Various kitchen tools and their use for food and meal preparation are described and illustrated for cooks. Included under the tool description category are: hands for kneading dough; knives; scissors and shears, other cutting tools; tools for poun...

Harris, JH., 1985: Age of Australian bass, Macquaria novemaculeata Perciformes Percichthyidae, in the Sydney Basin. The age of M. novemaculeata from the Hawkesbury River and other streams in the Sydney Basin, New South Wales, was determined using otoliths. Annuli were counted after simple sectioning of otoliths and intensification of the growth-check pattern by...

Foote, J.; Ziller, L., 1984: So why not start a foundation?. Foundation News 25(3): 50-58