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Type V phosphodiesterase expression in cerebral arteries with vasospasm after subarachnoid hemorrhage in a canine model

, : Type V phosphodiesterase expression in cerebral arteries with vasospasm after subarachnoid hemorrhage in a canine model. Neurological Research 24(6): 607-612

Cyclic GMP (cGMP) mediates smooth muscle relaxation in the central nervous system. In subarachnoid hemorrhage (SAH), decreases in intrinsic nitric oxide (NO) cause cerebral vasospasms due to the regulation of cGMP formation by NO-mediated pathways. As phosphodiesterase type V (PDE V) selectively hydrolyzes cGMP, we hypothesized that PDE V may function in the initiation of vasospasm. This study sought to identify the altered PDE V expression and activity in the vasospastic artery in a canine SAH model. We also used this system to examine possible therapeutic strategies to prevent vasospasm. Using a canine model of SAH, we induced cerebral vasospasm in the basilar artery (BA). Following angiographic confirmation of vasospasm on day 7, PDE V expression was immunohistochemically identified in smooth muscle cells of the vasospastic BA but not in cells of a control artery. The isolation of PDE enzymes using a sepharose column confirmed increased PDE V activity in the vasospastic artery only through both inhibition studies, using the highly selective PDE V inhibitor, sildenafil citrate, and Western blotting. Preliminary in vivo experiment using an oral PDE V inhibitor at 0.83 mg kg(-1) demonstrated partial relaxation of the spastic BA. PDE V activity was increased from control levels within the BA seven days after SAH. PDE V expression was most prominent in smooth muscle cells following SAH. These results suggest that clinical administration of a PDE V inhibitor may be a useful therapeutic tool in the prevention of vasospasm following SAH.


PMID: 12238630

DOI: 10.1179/016164102101200447

Other references

Fedorova G.I., 1970: Production of various forms of l growth by spheroplasts. Antibiotiki (moscow): 240-243

Walsh, P.O.-Leary, P., 1989: Community-based waste recycling--how to get started. Publication University of Wisconsin Cooperative Extension Service: (B3444)

Ebrid, B.; Ariño, M.D.; Cabeza, F.; Sebastián, A., 1992: Severe adverse reaction during treatment with rifampicin. Revista Clinica Espanola 191(4): 231-232

Regnier, M.; Morris, C.; Homsher, E., 1994: The effect of 2,3 butanedione monoxime on the crossbridge transition from weakly to strongly bound states in skinned rabbit skeletal muscle fibers. Biophysical Journal 66(2 PART 2): A191

Dodgshun, T.J.; Taylor, M.D.; Forrest, B.M., 2007: Human-mediated pathways of spread for non-indigenous marine species in New Zealand. Knowledge of human-mediated transport pathways for non-indigenous marine species (NIMS) to and around New Zealand is important for understanding and managing bioinvasion risks to high-value areas (HVAs). This document describes potential pathways...

Souquet, R., 1963: Isolated obliteration of the cubital artery at the wrist. Annales de Chirurgie Plastique 8: 103-103

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Guo, Y.; Luo, X.; Yu, M.; Zheng, L., 2011: Active ingredients and efficacies of Ganoderma lucidum cultivated on non-medicinal parts of Chinese medicinal herbs. Ganoderma lucidum was cultivated on non-medicinal parts of Salvia miltiorrhiza, Chrysanthemum morifolium, Ptatycodgn grandlfiorum, as all are Chinese traditional herbal medicines. We studied the changes of active ingredients and efficacies of the...

Ravise A.; Trique B., 1971: The determination of pectinolytic enzymes of 2 strains of phytophthora activity variations in the tissues of tomato seedlings in relation to the genomes of resistance. Annales de Phytopathologie 3(4): 529

Chou J.T., 1962: Stratigraphic and sedimentary study of the Mushan Formation in northern Taiwan. The Mushan formation is well known as one of the important coal-bearing formations of the Miocene sequence in Taiwan. This formation in northern Taiwan is thicker in the southeastern part, the thickest accumulation exceeding 670 meters, and thins...