geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Xenon preserves neutrophil and monocyte function in human whole blood


, : Xenon preserves neutrophil and monocyte function in human whole blood. Canadian Journal of Anaesthesia 49(9): 942-945

Purpose: Most volatile anesthetics are known to inhibit the oxidative and phagocytic function of neutrophils. In the present study, we investigated the effect of xenon on phagocytosis and respiratory burst activity of neutrophils and monocytes in human whole blood. Methods: Heparinized whole blood from 22 healthy volunteers was incubated for 60 min in the presence of 65% xenon. Sixty-five percent nitrous oxide was used as a positive control to prove the reliability of our in vitro system. Phagocytosis of fluorescein isothiocyanate labelled, opsonized Escherichia coli (E. coli) by neutrophils and monocytes was measured using flow cytometry. After induction with either N-formyl-methionyl-leucyl-phenylalanine (FMLP), phorbol-12-myristate-13-acetate or opsonized E. coli, respiratory burst activity was assessed by measuring the oxidation of dihydrorhodamine 123 to rhodamine 123 with a flow cytometer. Results: Exposure of human whole blood to xenon increased the percentage of nelftrophils showing phagocytosis (94+-3% vs 92+-4%; P<0.01), and the amount of ingested bacteria (P<0.01). Respiratory burst activity in neutrophils and monocytes was not affected by xenon. Nitrous oxide significantly reduced the percentage of neutrophils showing respiratory burst after FMLP stimulation. Furthermore, E. coli-induced stimulation resulted in a decreased number of reacting neutrophils (84+-15% vs 95+-5%; P<0.05) and monocytes (70+-22% vs 83+-11%; P<0.05) as well as a reduced production of hydrogen peroxide in both cell lines compared to control. Conclusion: In contrast to nitrous oxide, xenon preserves neutrophil and monocyte antibacterial capacity in vitro.

US$19.90

PMID: 12419721

DOI: 10.1007/BF03016879


Other references

Huber, C.A.; Pflüger, V.; Hamid, A-Wahab.M.; Forgor, A.A.; Hodgson, A.; Sié, A.; Junghanss, T.; Pluschke, G., 2013: Lack of antigenic diversification of major outer membrane proteins during clonal waves of Neisseria meningitidis serogroup A colonization and disease. In particular in the 'meningitis belt' of sub-Saharan Africa, epidemic meningococcal meningitis is a severe public health problem. In the past decades, serogroup A lineages have been the dominant etiologic agents, but also other serogrou...

Anonymous, 1981: WHO/WSAVA guidelines to reduce human health risks associated with animals in urban areas. These guidelines have been compiled to enable public health and veterinary authorities to set up health strategies to reduce the risks of zoonotic diseases, and injury, pollution or nuisance caused by animals in urban areas. Aspects covered includ...

Robain H.; Tessier D.; Grimaldi M.; Elsass F., 1990: Importance of kaolinite texture in the characterization of ferrallitic cover; consequences for physical stability and organization of soils. In ferrallitic soils from French Guyana, size and shape of kaolinite crystallites are pedogenetic features. Those steric parameters allow a better comprehension of particle size analysis. They also show the contribution of both crystallite fabric...

Nicks, B.; Debliquy, P.; Canart, B.; Buzitu, S.; Dewaele, A., 1992: A study on the changes in temperatures measured in 25 compartments in farrowing and weaning houses. Temp. was measured for 7 days, every 20 min., in 20 farrowing houses and 5 weaning houses. The mean stocking rate in the farrowing houses was 70% and the air space per litter (1 sow and 8 piglets) 25 msuperscript 3. In the weaning houses the mean...

Thomas, H.G., 1976: Provider's report on Indiana's Medicaid dental program. Journal 55(4): 8-12

Van der Heyden, J.; Van Neerven, D.; Sonker, U.; Bal, E.T.; Kelder, J.C.; Plokker, H.W.M.; Suttorp, M.J., 2012: Carotid artery stenting and cardiac surgery in symptomatic patients. The purpose of this study was to evaluate the feasibility and safety of the combined outcome of carotid artery stenting (CAS) and coronary artery bypass graft (CABG) surgery in neurologically symptomatic patients.The risk of perioperative stroke i...

Ma, C.; Chhikara, S.; Minocha, R.; Long, S.; Musante, C.; White, J.C.; Xing, B.; Dhankher, O.Parkash., 2016: Reduced Silver Nanoparticle Phytotoxicity in Crambe abyssinica with Enhanced Glutathione Production by Overexpressing Bacterial γ-Glutamylcysteine Synthase. Silver nanoparticles (Ag NPs) are widely used in consumer products, and their release has raised serious concerns about the risk of their exposure to the environment and to human health. However, biochemical mechanisms by which plants counteract N...

Sakamoto, T.; Tanaka, T.; Itoi, H.; Yoshihara, M., 1999: Stopper mechanism for endoscopically inserting ultrasound probe. A stopper mechanism for an endoscopically insertable ultrasound probe which is designed to be introduced into a body cavity through a biopsy channel provided internally of an insertion instrument of an endoscope and which is of the type having a b...

Kramer, U.; Despland, J-Nicolas.; Michel, L.; Drapeau, M.; de Roten, Y., 2011: Change in defense mechanisms and coping over the course of short-term dynamic psychotherapy for adjustment disorder. Short-term dynamic psychotherapy (STDP) has rarely been investigated with regard to its underlying mechanisms of change, even if psychoanalytic theory informs us about several potential putative mechanisms of change in patients. Change in overall...

Lawrey, J.D.; Lücking, R.; Sipman, H.J.M.; Chaves, Jé.L.; Redhead, S.A.; Bungartz, F.; Sikaroodi, M.; Gillevet, P.M., 2010: High concentration of basidiolichens in a single family of agaricoid mushrooms (Basidiomycota: Agaricales: Hygrophoraceae). The Agaricales is the largest and most diverse order of mushroom-forming Basidiomycota, with over 100 natural groups recognized in recent Fungal Tree of Life studies. Most agarics are either saprotrophic or ectomycorrhizal fungi, but the family Hy...