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Beta(2)-adrenergic and several other G protein-coupled receptors in human atrial membranes activate both G(s) and G(i)

, : Beta(2)-adrenergic and several other G protein-coupled receptors in human atrial membranes activate both G(s) and G(i). Circulation Research 87(8): 705-709

Cardiac G protein-coupled receptors that couple to Galpha(s) and stimulate cAMP formation (eg, beta-adrenergic, histamine, serotonin, and glucagon receptors) play a key role in cardiac inotropy. Recent studies in rodent cardiac myocytes and transfected cells have revealed that one of these receptors, the beta(2)-adrenergic receptor (AR), also couples to the inhibitory G protein Galpha(i) (activation of which inhibits cAMP formation). If beta(2)ARs could be shown to couple to Galpha(i) in the human heart, it would have important ramifications, because levels of Galpha(i) increase with age and in failing human heart. Therefore, we investigated whether beta(2)ARs in the human heart activate Galpha(i). By photoaffinity labeling human atrial membranes with [(32)P]azidoanilido-GTP, followed by immunoprecipitation with antibodies specific for Galpha(i), we found that Galpha(i) is activated by stimulation of beta(2)ARs but not of beta(1)ARs. In addition, we found that other Galpha(s)-coupled receptors also couple to Galpha(i), including histamine, serotonin, and glucagon. When coupling of these receptors to Galpha(i) is disrupted by pertussis toxin, their ability to stimulate adenylyl cyclase is enhanced. These data provide the first evidence that beta(2)AR and many other Galpha(s)-coupled receptors in human atrium also couple to Galpha(i) and that abolishing the coupling of these receptors to Galpha(i) increases the receptor-mediated adenylyl cyclase activity.


PMID: 11029407

Other references

Bijlenga R.K.L.; Broek R.V.D.; Kellenberger E., 1974: The transformation of tau particles into phage t 4 heads part 1 evidence for the conservative mode of this transformation. Journal of Supramolecular Structure 2(1): 45-59

Weismann, A., 1881: Beobachtungen an Hydroid-Polypen. I. Pulsiren des Korperschlauchs. II. Selbstandige Bewegungen des Ectoderms. From observations first made on Coryne pusilla, it appears that distinct rhythmical waves of contraction pass down the body-walls, which would assist the action of the cil...

Guillaume, M-C.; Peypouquet, J-P.; Tetart, J., 1985: Quaternaire et actuel. Bulletin Centre de Recherches Exploration-Production Elf-Aquitaine Memoire, 337-377 No. 9

Lie, H.; Malde, K.; Sorteberg, K.; Hasvold, O.; Ekren, T.; Malm, O.J., 1982: Long-term fluoride and calcium therapy of postmenopausal osteoporosis. In Norway, 12 ambulatory women 54 to 82 years old with symptomatic primary osteoporosis were treated for 2 years with 40 mg fluoride (88 mg NaF) daily in 2 divided doses with the morning and evening meal. Sodium fluoride was given as a sustained r...

Nayudu M.; Bender G.L.; Bassam B.J.; Sinclair M.; Rolfe B.G., 1987: Identification of host specificity dna regions determining the broad host range modulation of rhizobium strain ngr234. Verma, D P S And N Brisson (Ed ) Current Plant Science And Biotechnology in Agriculture: Molecular Genetics Of Plant-Microbe Interactions; Third International Symposium, Montreal, Quebec, Canada, July 27-31, 1986 Xxxii+338p Kluwer Academic Publishers: Dordrecht, Netherlands; Boston, Massachusetts, Usa Illus 188-190

Philpotts John A.; Taylor Cliff D.; Evans John R.; Emsbo Poul, 1993: Newly discovered molybdenite occurrences at Dora Bay, Prince of Wales Island, Southeast Alaska, and preliminary scanning electron microscope studies. U S

Sechzer, J.A.; Rabinowitz, V.C.; Denmark, F.L.; McGinn, M.F.; Weeks, B.M.; Wilkens, C.L., 1994: Sex and gender bias in animal research and in clinical studies of cancer, cardiovascular disease, and depression. Annals of the New York Academy of Sciences 736: 21-48

Kageyama, T.; Kimoto, S.; Funagoshi, K.; Sugiyama, S.; Tsumoto, S., 1975: Aplastic anemia and labor. Bulletin of the Osaka Medical School 21(1): 19-27

Peloso, J.G.; Mundy, G.D.; Cohen, N.D., 1994: Prevalence of, and factors associated with, musculoskeletal racing injuries of thoroughbreds. All horses diagnosed by a commission veterinarian of the Kentucky Racing Commission as having sustained a musculoskeletal injury, defined as an obvious change in soundness immediately before, during, or after a race held between Jan 1, 1992 and Ma...

Tong, M.H.; Zohar, O.; Hayhoe, M.M., 2017: Control of gaze while walking: Task structure, reward, and uncertainty. While it is universally acknowledged that both bottom up and top down factors contribute to allocation of gaze, we currently have limited understanding of how top-down factors determine gaze choices in the context of ongoing natural behavior. One...