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A versatile method of identifying specific binding proteins on affinity resins

, : A versatile method of identifying specific binding proteins on affinity resins. Analytical Biochemistry 352(1): 15-23

The isolation of both specific and nonspecific binding proteins on affinity matrices bearing bioactive compounds hinders the identification of drug cellular targets. Although solid-phase elution and competition methods conventionally are used to distinguish between specific and nonspecific receptor-ligand interactions, these approaches often are severely restricted by low ligand solubility and/or slow kinetic dissociation. This article describes an alternative and versatile method, termed serial affinity chromatography, to identify ligand receptors using affinity resins. (c) 2006 Elsevier Inc. All rights reserved.


PMID: 16540075

DOI: 10.1016/j.ab.2006.02.008

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