+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Acute induction of human IL-8 production by intestinal epithelium triggers neutrophil infiltration without mucosal injury

, : Acute induction of human IL-8 production by intestinal epithelium triggers neutrophil infiltration without mucosal injury. Gut 54(11): 1565-1572

Aim: Neutrophil migration in the intestine depends on chemotaxis of neutrophils to CXC chemokines produced by epithelial cells. The goal of this project was to determine if acute induction of a CXC chemokine gradient originating from intestinal epithelial cells is sufficient to induce neutrophil influx into intact intestinal tissue.Methods and results: The authors developed a double transgenic mouse model with doxycycline induced human IL-8 expression restricted to intestinal epithelial cells. Doxycycline treatment of double transgenic mice for three days resulted in a 50-fold increase in the caecal IL-8 concentration and influx of neutrophils into the lamina propria. Although neutrophils entered the paracellular space between epithelial cells, complete transepithelial migration was not observed. Doxycycline treatment also increased the water content of the caecal and colonic stool, indicating dysfunctional water transport. However, the transmural electrical resistance was not decreased. Neutrophils recruited to the intestinal epithelium did not show evidence of degranulation and the epithelium remained intact as judged by histology.Conclusions: This conditional transgenic model of chemokine expression provides evidence that acute induction of IL-8 in the intestinal epithelium is sufficient to trigger neutrophil recruitment to the lamina propria, but additional activation signals are needed for full activation and degranulation of neutrophils, mucosal injury, and complete transepithelial migration.


PMID: 15987794

DOI: 10.1136/gut.2004.061168

Other references

Kukovskiy Y.G.; Lagali G.; Vayss A., 1971: Determination of the layer charge in montmorillonite. Mineralogicheskiy Sbornik (L'vov) 25, Part 1: 11-17

Fister, S.; Robben, C.; Witte, A.K.; Schoder, D.; Wagner, M.; Rossmanith, P., 2016: Influence of Environmental Factors on Phage-Bacteria Interaction and on the Efficacy and Infectivity of Phage P100. When using bacteriophages to control food-borne bacteria in food production plants and processed food, it is crucial to consider that environmental conditions influence their stability. These conditions can also affect the physiological state of b...

Munoz-Garcia, D.; Ludwin, S.K., 1986: Gliogenesis in organotypic tissue culture of the spinal cord of the embryonic mouse. I. Immunocytochemical and ultrastructural studies. The technique of organotypic tissue culture offers an opportunity to observe in vitro complex interactions among glial cells and neurons, leading to the formation of myelin. In the present and accompanying work a combined ultrastructural, immunocy...

Howat, A.J.; Armstrong, G.R.; Briggs, W.A.; Nicholson, C.M.; Stewart, D.J., 1992: Fine needle aspiration of palpable breast lumps: a 1-year audit using the Cytospin method. A fine-needle aspiration (FNA) service for the diagnosis of palpable breast lumps was started at the Royal Preston Hospital, Preston, UK, in November 1989. Over the subsequent year, 407 FNAs were taken from 393 women. A simple technique was used w...

Chen, D.; Zhang, K.; Li, Y.; Li, F.; Hu, Z.; Li, X., 2001: Effects of dietary supplementation of copper and vitamin E on pigmeat quality. Journal of Dairy Science 84(Supplement 1): 19

Bistrian, B.R., 2017: Protein calorie malnutrition and obesity: Nutritional collaboration from MIT to the bedside and clinic. Metabolism: Clinical and Experimental

Luria, S.; Givoni, S.; Heled, Y.; Tadmor, B.; Khanin, A.; Epstein, Y., 2004: Evaluation of CO2 accumulation in respiratory protective devices. A simple mathematical model was developed to grade the results of the fraction of inspiratory CO2 levels in respiratory protective devices (RPDs). Using this model, the level of FiCO2 of two chemical warfare RPDs was monitored during the examinati...

Kohrt, W.M.; Wierman, M.E., 2017: Preventing Fat Gain by Blocking Follicle-Stimulating Hormone. New England Journal of Medicine 377(3): 293-295

Riley, C.; Felvus, J.; Warner, M.; Greenacre, J., 1992: Over the obstacles. Health Service Journal 102(5294): 26-27

Smith, J.T.; Acohido, B.V.; Clifton, D.K.; Steiner, R.A., 2006: KiSS-1 neurones are direct targets for leptin in the ob/ob mouse. Leptin is an adipocyte-derived hormone that acts on the hypothalamus to influence feeding, metabolism and reproduction, but the cellular and molecular targets for the action of leptin in the brain have yet to be fully elucidated. Kisspeptins are e...