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Adapter-ligation mediated allele-specific amplification (ALM-ASA) for multiplex SNP genotyping

, : Adapter-ligation mediated allele-specific amplification (ALM-ASA) for multiplex SNP genotyping. Yi Chuan 28(2): 219-225

To establish adapter-ligation mediated allele-specific amplification ("ALM-ASA" for short) for multiplex SNP genotyping, five SNPs, 100C > T, 1661G > C, 1758G > T, 2470T > C and 2850C > T in CYP2D6 gene were used as an example for evaluating the method. Firstly, a preamplification was carried out for producing a long target containing all SNPs of interest. Secondly, the preamplified DNA fragments were digested with a restriction endonuclease to form sticky ends.


PMID: 16520320

Other references

Basalamah, A.H.; Serebour, F.E.K., 1981: Toxoplasmosis in pregnancy. A survey of 1000 pregnant Saudis and non-Saudis attending King Abdulaziz University Hospital, Jeddah. Toxoplasma gondii, human, infections during pregnancy, incidence survey of 1000 patients, general clinical review: Jeddah, Saudi Arabia

Mir, T.A.; Reddy, A.K.; Burkholder, B.M.; Walsh, J.; Shifera, A.Selassie.; Khan, I.R.; Thorne, J.E., 2017: Clinical Features and Incidence Rates of Ocular Complications in Patients With Retinal Vasculitis. To describe the incidence rates of visual loss and ocular complications in patients with retinal vasculitis (RV). Retrospective cohort study. Clinical data were collected for 96 patients (175 eyes) diagnosed with RV from 2003 to 2013. Main outcome...

Krutzsch Wilfried, 1967: Changes in the early Tertiary flora of central Europe on the basis of palynologic investigations. Pollen zones in Paleocene-middle Oligocene, correlation with marine sequences of North Sea basin, paleoclimatic effects, minimum floras (at beginning and end of Paleocene), maximum floras (middle Paleocene, Eocene).

Teixeira, C.R.; Tanaka, S.; Haruma, K.; Yoshihara, M.; Sumii, K.; Kajiyama, G.; Shimamoto, F., 1996: Flat-elevated colorectal neoplasms exhibit a high malignant potential. The potential of flat-elevated colorectal adenomas to undergo rapid malignant transformation and progression to invasive carcinoma is still under discussion. Therefore, a total of 130 colorectal neoplastic lesions > or = 1 cm in diameter were e...

Solerte, S.B.; Fioravanti, M., 2002: Bood rheology, inflammation and Alzheimers Disease Hypothesis in neurotoxic mechanism and basis for rheological treatment. Biorheology 39(5): 619

Kramer, L.; Alberts, E.; Ghidey, F., 1991: Validation of WEPP 91.2 with erosion plot data. American Society of Agricultural Engineers Microfiche collection: (fiche no 91-2079)

Ekström, N.; Ahman, H.; Verho, J.; Jokinen, J.; Väkeväinen, M.; Kilpi, T.; Käyhty, H., 2004: Kinetics and avidity of antibodies evoked by heptavalent pneumococcal conjugate vaccines PncCRM and PncOMPC in the Finnish Otitis Media Vaccine Trial. The licensure of new pneumococcal conjugate vaccines (PCVs) relies on immunogenicity data. When defining correlates of protection, vaccine efficacy data must be included. In the FinOM Vaccine Efficacy Trial, the PncOMPC vaccine showed an efficacy...

Koch, K.S.; Maeda, S.; He, G.; Karin, M.; Leffert, H.L., 2009: Targeted deletion of hepatocyte Ikkbeta confers growth advantages. Mice lacking hepatocyte IKKbeta (Ikkbeta(Delta hep)) are defective in TNFalpha-activation of hepatocellular transcription factor NF-kappaB, and highly susceptible to hepatotoxicity. Following diethylnitrosamine (DEN) exposure, Ikkbeta(Delta hep) m...

Duncan, W.C.; Mcbride, M.E.; Knox, J.M., 1970: Experimental production of infections in humans. Journal of Investigative Dermatology 54(4): 319-323

Anonymous, 2002: Cardiac scoring. Clinical Privilege White Paper: 1-8