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Adapter-ligation mediated allele-specific amplification (ALM-ASA) for multiplex SNP genotyping


, : Adapter-ligation mediated allele-specific amplification (ALM-ASA) for multiplex SNP genotyping. Yi Chuan 28(2): 219-225

To establish adapter-ligation mediated allele-specific amplification ("ALM-ASA" for short) for multiplex SNP genotyping, five SNPs, 100C > T, 1661G > C, 1758G > T, 2470T > C and 2850C > T in CYP2D6 gene were used as an example for evaluating the method. Firstly, a preamplification was carried out for producing a long target containing all SNPs of interest. Secondly, the preamplified DNA fragments were digested with a restriction endonuclease to form sticky ends.

US$29.90

PMID: 16520320


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