+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Adjudin-mediated junction restructuring in the seminiferous epithelium leads to displacement of soluble guanylate cyclase from adherens junctions

, : Adjudin-mediated junction restructuring in the seminiferous epithelium leads to displacement of soluble guanylate cyclase from adherens junctions. Journal of Cellular Physiology 208(1): 175-187

A plethora of evidence supports the role of cyclic nucleotides injunction restructuring. For instance, studies have shown cGMP to be a key regulator of junction assembly and disassembly in different in vitro and in vivo systems. In this study, we examine the role of soluble guanylate cyclase (sGC) in junction restructuring in the seminiferous epithelium of the rat testis. First, the interaction of soluble guanylate cyclase beta 1 (sGC beta 1; sGC is a heterodimer comprised of an a and a P subunit) with proteins that constitute adherens and tight junctions in the testis was demonstrated. By immunoprecipitation, sGC beta 1 was found to associate with occludin, JAM-A, and ZO-1, as well as with cadherin, catenin, nectin, afadin, ponsin, and espin, suggestive of its role in cell junction dynamics. These results were corroborated in part by immunohistochemistry experiments, which revealed that the localization of sGC beta 1 was largely restricted to the site of the apical and basal ectoplasmic specialization. Next, the role of sGC injunction dynamics was addressed by using an in vivo model of junction restructuring. Administration of Adjudin - a chemical entity known to specifically perturb adhesion between Sertoli and germ cells (i.e., round and elongate(ing) spermatids and most spermatocytes) - resulted in a similar to 1.5-fold increase in sGC beta 1, coinciding with the loss of germ cells from the epithelium. More importantly, the ability of sGC beta 1 to associate with cadherin increased approximately three-fold during Adjudin-mediated restructuring of Sertoli-germ cell junctions, whereas its interaction with tight junction proteins (i.e., occludin and ZO-1) decreased. Taken collectively, these results suggest that sGC participates in the remodeling of cell junctions during spermatogenesis.


PMID: 16547975

DOI: 10.1002/jcp.20651

Other references

Shin, B.C.; Chung, J.H.; Kim, H.L., 2014: Sirolimus: a switch option for mycophenolate mofetil-induced leukopenia in renal transplant recipients. Mycophenolate mofetil (MMF) is a potent immunosuppressive agent used to prevent acute and chronic rejection in kidney transplantation or for rescue therapy. One side effect of MMF is bone marrow toxicity, including leukopenia, which may necessitat...

Ma; Boltich; Sankar; Wallace, 1989: Effect of texture and spin reorientation on the magnetic properties of Nd-Fe-B magnets. Physical Review. B, Condensed Matter 40(10): 7332-7335

Bonabeau, E.; Théraulaz, G., 2000: Swarm smarts. Scientific American 282(3): 72-79

Kaya, M.G.; Ozkan, M.; Gunebakmaz, O.; Akkaya, H.; Kaya, E.G.; Akpek, M.; Kalay, N.; Dikilitas, M.; Yarlioglues, M.; Karaca, H.; Berk, V.; Ardic, I.; Ergin, A.; Lam, Y.Yin., 2014: Protective effects of nebivolol against anthracycline-induced cardiomyopathy: a randomized control study. We aimed to evaluate the effect of prophylactic nebivolol use on prevention of antracycline-induced cardiotoxicity in breast cancer patients. In this small, prospective, double-blind study, we randomly assigned 45 consecutive patients with breast...

Ericsson, O.; Sivertsson, A.; Lundeberg, J.; Ahmadian, A., 2003: Microarray-based resequencing by apyrase-mediated allele-specific extension. We have developed an array-based resequencing method to determine genetic alterations in putative cancer genes. The method relies on that the specificity of DNA polymerase in allele-specific extensions can be enhanced by terminating the extension...

Tomiyama H.; Carretero O.A.; Scicli G.M.; Scicli A.G., 1989: Changes in renal function induced by fab fragments of bradykinin antibodies. FASEB Journal 3(4): A1027

Schwarzenberger, K.; Udey, M.C., 1996: Contact allergens and epidermal proinflammatory cytokines modulate Langerhans cell E-cadherin expression in situ. After exposure to antigen, Langerhans cells (LC) migrate from the epidermis to lymph nodes, where they initiate primary immune responses in T cells. The adhesion molecule E-cadherin mediates adhesion of LC to keratinocytes in vitro and may be resp...

Santos, A.-Dos; Athayde, J., 1983: Ditylenchus dipsaci (Kuhn) Filipjev in garlic in the state of Espirito Santo. Revista ceres 30(172): 477-480

Agacayak, E.; Tunc, S.Yaman.; Sak, S.; Basaranoglu, S.; Yüksel, H.; Turgut, A.; Gul, T., 2016: Levels of Neopterin and other Inflammatory Markers in Obese and Non-Obese Patients with Polycystic Ovary Syndrome. We aimed to measure the levels of inflammatory markers and neopterin in obese and non-obese patients with PCOS by using 2 separate control groups with matching body mass index (BMI). A total of 60 women of reproductive age with (n=30) and without...

Thomson A.M.; Lodge D., 1985: Selective blockade of an excitatory synapse in rat cerebral cortex by the sigma opiate cyclazocine an intracellular in vitro study. In isolated slices of rat cortex, intracellularly recorded responses of pyramidal neurons to N-methylaspartate (NMA) and glutamate and responses to stimulation of the underlying white matter were compared and challenged with the sigma opiate cycla...