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An improved recombineering approach by adding RecA to lambda red recombination


, : An improved recombineering approach by adding RecA to lambda red recombination. Molecular Biotechnology 32(1): 43-53

Recombineering is the use of homologous recombination in Escherichia coli for DNA engineering. Of several approaches, use of the, lambda phage Red operon is emerging as the most reliable and flexible. The Red operon includes three components: Red alpha, a 5' to 3' exonuclease, Red beta, an annealing protein, and Red gamma, an inhibitor of the major E. coli exonuclease and recombination complex, RecBCD.

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