+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Analysis of the Xenopus Werner syndrome protein in DNA double-strand break repair

, : Analysis of the Xenopus Werner syndrome protein in DNA double-strand break repair. Journal of Cell Biology 171(2): 217-227

Werner syndrome is associated with premature aging and increased risk of cancer. Werner syndrome protein (WRN) is a RecQ-type DNA helicase, which seems to participate in DNA replication, double-strand break (DSB) repair, and telomere maintenance; however, its exact function remains elusive. Using Xenopus egg extracts as the model system, we found that Xenopus WRN (xWRN) is recruited to discrete foci upon induction of DSBs. Depletion of xWRN has no significant effect on nonhomologous end-joining of DSB ends, but it causes a significant reduction in the homology-dependent single-strand annealing DSB repair pathway. These results provide the first direct biochemical evidence that links WRN to a specific DSB repair pathway. The assay for single-strand annealing that was developed in this study also provides a powerful biochemical system for mechanistic analysis of homology-dependent DSB repair.


PMID: 16247024

DOI: 10.1083/jcb.200502077

Other references

Yaziji, H.; Sneige, N.; Gown, A.M., 2000: Comparative sensitivities and specificities of myoepithelial markers in the detection of stromal invasion in breast cancer. Laboratory Investigation 80(3): 35A, March

Hofer S.; Ingold P., 1984: The whistles of the alpine marmot marmota marmota marmota their structure and occurrence in the antipredator context. The whistles of the alpine marmot given in front of different predators and the responses of conspecifics to them are described. Observations and recordings performed in 1979 and 1980 in the Bernese Alps [Switzerland] led to the following results:...

Haines, D.E.; Cajal, S.Ramón., 2007: Santiago Ramón y Cajal at Clark University, 1899; his only visit to the United States. In 1899, at the invitation of G. Stanley Hall, the great psychologist and President of Clark University, Santiago Ramón y Cajal, and four other European scientists of significant note, were invited to participate in the Decennial Celebration of C...

Samvelyan V.M., 1968: Effect of the adrenal cortex on the development and experimental therapy of edema swelling of the rat brain. Patologicheskaya Fiziologiya i Eksperimental'naya Terapiya 12(1): 45-48

Huet-Buffet, S., 2005: Cancer, towards a new management. You will tell them.... Soins; la Revue de Reference Infirmiere: 44-45

Coca-Prados, M.; Hsu, M.T., 1979: Intracellular forms of simian virus 40 nucleoprotein complexes. II. Biochemical and electron microscopic analysis of simian virus 40 virion assembly. The simian virus 40 virion assembly process was studied with pulse-labeling kinetics of virion proteins, CsCl gradient analysis, electron microscopy, and low-salt gel electrophoresis. The results obtained are consistent with the model of gradual a...

Aronov, A.M.; Aronov, E.A.; Belik, D.V., 2007: Improvement of organization and economic mechanisms for comprehensive provision of equipment to treatment-and-prophylaxis institutions. In many domestic treatment-and-prophylaxis institutions, the standard service life of the majority of medical equipment already expired. Further use of this equipment can become hazardous to both patients and medical personnel. Medical techniques...

Karlen D.L.; Berti W.R.; Hunt P.G.; Matheny T.A., 1989: Soil test values after eight years of tillage research on a norfolk loamy sand. Long-term effects of alternate tillage systems on soil-test values for Coastal Plain soils were unknown. Therefore, soil pH, organic carbon, and Mehlich I extractable P, K, Ca, and Mg concentrations measured during an eight-year tillage study on N...

Pontin, D.; Lewis, M., 2007: Managing the caseload: a qualitative action research study exploring how community children's nurses deliver services to children living with life-limiting, life-threatening, and chronic conditions. PURPOSE; The number of children living with life-limiting, life-threatening, and chronic conditions nursed at home/in the community is increasing. There is limited literature on how community children's nurses (CCNs) manage their caseloads. A...

Eleftheriadis, K.; Balis, D.; Ziomas, I.C.; Colbeck, I.; Manalis, N., 1998: Atmospheric aerosol and gaseous species in Athens, Greece. Measurements of aerosol species including Cl-, NO3-, SO42-, NH4+, Na+, K+, Mg2+ and Ca2+ and gaseous pollutants like HCl, HNO3, NO2, SO2 and O3 were conducted at the centre of Athens. Results show moderate levels of pollution with aerosol species...