+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Analysis of the cytogenetic stability of the human embryonal kidney cell line 293 by cytogenetic and STR profiling approaches

, : Analysis of the cytogenetic stability of the human embryonal kidney cell line 293 by cytogenetic and STR profiling approaches. Cytogenetic and Genome Research 106(1): 28-32

We have characterized the cytogenetic alterations of the human embyronal cell line 293 by spectral karyotyping and G-banding analysis. To investigate its genomic stability, we compared the karyotypes of 293 and its daughter line EcR-293. Genotype profiling through short tandem repeats complemented the analysis. While displaying almost identical STR profiles and thus verifying their origin and their close relation, the two lines were remarkably different in their number of chromosomes and setup of aberrant chromosomes. However, the cell lines retained a stable karyotype in long term culture. The establishment of subclones from EcR-293, expressing inducible lacZ or MEN1 transgenes, only added minor changes to the karyotype. Our study shows that the cytogenetic constitution of a clonal cell line of the 293 origin appears to be sufficiently stable. However, care should be taken when comparing the properties of independent 293 lineages, since clonal variations might be substantial. Copyright (C) 2004 S. Karger AG, Basel.


PMID: 15218237

DOI: 10.1159/000078556

Other references

Borer, J.S., 1992: Advances in Cardiovascular Pharmacology: Protocol Design and Methodology. Proceedings of the 11th annual symposium. Atherosclerosis and Drug Development for Ischemic Heart Disease. Journal of Clinical Pharmacology 32(9): 848-866

Pushin, D.A.; Arif, M.; Huber, M.G.; Cory, D.G., 2008: Measurements of the vertical coherence length in neutron interferometry. The study and use of macroscopic quantum coherence requires long coherence lengths. Here we describe an approach to measuring the vertical coherence length in neutron interferometry, along with improvements to the NIST interferometer that led to a...

Copeland, K.C., 1988: Effects of acute high dose and chronic low dose estrogen on plasma somatomedin-C and growth in patients with Turner's syndrome. Previously reported data with regard to the effects of estrogen on plasma somatomedin-C (Sm-C) concentrations are contradictory. This study was designed to evaluate, in the same subjects with Turner's syndrome, the effects of both acute high...

Luckey, T.D.; Pleasants, J.R.; Wagner, M.; Gordon, H.A.; Reyniers, J.A., 1955: Some observations on vitamin metabolism in germ-free rats. Limited numbers of germ-free albino rats were fed semi-synthetic diets in experiments designed to give the maximum amount of knowledge about the vitamin requirements of this mammal reared in the complete absence from all forms of living organisms...

Schneider, L.G.; Jackson, H.C.; Hohnsbeen, K.P., 1981: Annual summary of rabies in Europe, 1981. A total of 18 549 rabies cases were reported in 18 of the 26 countries participating in the European Rabies Surveillance System--an increase of 5.1% on 1980. There were 4788 cases (24.5% of total) in domestic animals, 14 759 (75.5%) in wildlife, a...

Zeylik B.S., 1975: The Balkhash-Ili cosmogenic structure and prospects for copper-nickel mineralization in the Balkhash region. Doklady Akademii Nauk SSSR: 6, Pages 1410-1413. 1975.

Little, E.G., 1918: Case of Rodent Ulcer treated by Radium. Proceedings of the Royal Society of Medicine 11(Dermatol Sect): 63-64

Galler, A.; Shibly, S.; Bilek, A.; Hirt, R.A., 2014: Inhaled budesonide therapy in cats with naturally occurring chronic bronchial disease (feline asthma and chronic bronchitis). To describe the long term use of inhaled budesonide in cats with naturally occurring asthma and chronic bronchitis and to measure its effects. Owners of 43 cats diagnosed with asthma or chronic bronchitis, which had been prescribed 400 µg of in...

Mondovì, B.; Riccio, P., 1984: Copper amine oxidases: structure and function. Advances in Inorganic Biochemistry 6: 225-244

Rossi, A.; Pollice, R.; Daneluzzo, E.; Marinangeli, M.G.; Stratta, P., 2000: Behavioral neurodevelopment abnormalities and schizophrenic disorder: a retrospective evaluation with the Childhood Behavior Checklist (CBCL). This study is a retrospective report of childhood and adolescence neurobehavioral assessment in patients with schizophrenia and their healthy siblings using the Childhood Behavior Checklist (CBCL). The CBCL ratings were obtained from retrospective...