geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Analysis of the cytoplasmic domains of Salmonella FlhA and interactions with components of the flagellar export machinery


, : Analysis of the cytoplasmic domains of Salmonella FlhA and interactions with components of the flagellar export machinery. Journal of Bacteriology 186(22): 7586-7592

Most flagellar proteins are exported via a type III export apparatus which, in part, consists of the membrane proteins FlhA, FlhB, FliO, FliP, FliQ, and FliR and is housed within the membrane-supramembrane ring formed by FliF subunits. Salmonella FlhA is a 692-residue integral membrane protein with eight predicted transmembrane spans. Its function is not understood, but it is necessary for flagellar export. We have created mutants in which potentially important sequences were deleted. FlhA lacking the amino-terminal sequence prior to the first transmembrane span failed to complement and was dominant negative, suggesting that the sequence is required for function. Similar effects were seen in a variant lacking a highly conserved domain (FHIPEP) within a putative cytoplasmic loop. Scanning deletion analysis of the cytoplasmic domain (FlhAc) demonstrated that substantially all of FlhAc is required for efficient function. Affinity blotting showed that FlhA interacts with several other export apparatus membrane proteins. The implications of these findings are discussed, and a model of FlhA within the export apparatus is presented.

US$19.90

PMID: 15516571

DOI: 10.1128/JB.186.22.7586-7592.2004


Other references

Kopin I.J.; Berv K.R.; Webb J.G., 1973: Sympathetic ganglia in culture. Usdin, Earl And Solomon H Snyder (Ed ) Frontiers in Catecholamine Research: Proceedings Of The Third International Catecholamine Symposium Strasbourg, France, May 20-25, Xvi+1219p Illus Pergamon Press, Inc : New York, N Y , U S A ; London, England (1974)

Asturiol, D.; Lasorne, B.; Worth, G.A.; Robb, M.A.; Blancafort, Lís., 2010: Exploring the sloped-to-peaked S2/S1 seam of intersection of thymine with electronic structure and direct quantum dynamics calculations. The role of the seam of intersection between the lowest (pi,pi*) and (n,pi*) excited states in the decay of electronically excited singlet thymine has been investigated with ab initio complete active space self-consistent field (CASSCF) calculatio...

Closon, J.; Barac, G.; Hillen, E., 1956: Duodeno-intestinal absorption and urinary excretion of NaI 131 in dogs. Comptes Rendus des Seances de la Societe de Biologie et de Ses Filiales 150(8-9): 1633-1637

Motomura, K.; Le, Q.T.N.; Hamada, T.; Kutsuna, N.; Mano, S.; Nishimura, M.; Watanabe, Y., 2016: Diffuse decapping enzyme DCP2 accumulates in DCP1 foci under heat stress in Arabidopsis thaliana. The decapping enzymes DCP1 and DCP2 are components of a decapping complex that degrades mRNAs. DCP2 is the catalytic core and DCP1 is an auxiliary subunit. It has been assumed that DCP1 and DCP2 are consistently co-localized in cytoplasmic RNA gra...

Kujala, V., 1962: The depth of the moss layer in Finnish wooded heaths.. Gives the results of measurements of the two commonest mosses-Pleurozium schreberi and Hylocomium splendens.

Oelberg, D.G.; Xu, F., 1994: Diffusion potentials spontaneously develop in plasma membrane vesicles from type II pneumocytes. Pediatric Research 37(4 PART 2): 395A

Pamment, P.; Foenander, F.; McBride, G., 1983: Social and spatial organization of male behaviour in mated domestic fowl. Observations were made of male spacing, mating and agonistic behaviour in 2 pens of mated domestic fowl. In both pens, site attachment was found, with a bunching of observations for cocks living close to a wall. High-ranking males appeared to move...

Coombes, R., 2002: Workplace violence. Judge cracks down as violence soars. Nursing Times 96(30): 13-13

Burdett, L.; Smith, K.; Zhang, G.H.; Fernandez Vina, M., 2000: Resolution of B-locus Sequencing-Based Typing ambiguities. Human Immunology 61(Suppl 1): S5

Bers, D.M., 1982: A simple method for the accurate determination of free calcium concentration in calcium ethylene glycol bis beta aminoethyl ether n n n' n' tetra acetic acid solutions. A simple method for the accurate determination of free [Ca] in ethyleneglycol-bis(.beta.-aminoethylether)-N,N'-tetraacetic acid (EGTA)-buffered Ca solutions is described. This method is useful for calibration of Ca macro- and microelectrodes...