+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Assessment of DNA damage in peripheral blood of heavy smokers with the comet assay and the micronucleus test

, : Assessment of DNA damage in peripheral blood of heavy smokers with the comet assay and the micronucleus test. Mutation Research 581(1-2): 105-114

The comet assay (single-cell gel electrophoresis, SCG) is being increasingly used in human biomonitoring for the detection of genotoxic exposures. Cigarette smoking is a well-documented source of a variety of potentially mutagenic and carcinogenic compounds. Therefore, smoking should represent a relevant mutagenic exposure and lead to genotoxic effects in exposed cells. However, our previous investigations as well as several other published studies on human biomonitoring failed to show an effect of smoking on DNA migration in the comet assay, while some other studies did indicate such an effect. Although many factors can contribute to the generation of discrepant results in such studies, clear effects should be obtained after high exposure. We therefore performed a comparative study with healthy male heavy smokers (> 20 cigarettes per day) and non-smokers (n = 12 in each group). We measured the baseline comet assay effects in fresh whole blood samples and isolated lymphocytes. In addition, the amount of 'formamidopyrimidine DNA-glycosylase (FPG)-sensitive sites' was determined by a combination of the standard comet assay with the bacterial FPG protein. Furthermore, the influence of a repair inhibitor (aphidicolin, APC) on baseline DNA damage was comparatively analysed. Duplicate slides from each sample were processed and analysed separately. In all experiments, a reference standard (untreated V79 cells) was included to correct for assay variability. Finally, to compare the comet assay results with another genetic endpoint, all blood samples were investigated in parallel by the micronucleus test (MNT). Baseline and gamma radiation-induced micronucleus frequencies were determined. None of these approaches revealed a significant difference between heavy smokers and non-smokers with regard to a genotoxic effect in peripheral blood cells. (c) 2004 Elsevier B.V. All rights reserved.


PMID: 15725610

DOI: 10.1016/j.mrgentox.2004.11.015

Other references

Krapf F.; Renger D.; Schedel I.; Leiendecker K.; Leyssens H.; Deicher H., 1982: A poly ethylene glycol precipitation laser nephelometer technique for detection and characterization of circulating immune complexes in human sera. Based upon an automated laser nephelometric system, a rapid and sensitive technique was developed for quantitation and analysis of circulating immune complexes (CIC). Human serum samples were precipitated with 2.75% polyethylene glycol 6000 (PEG)....

Gillette, DD., 1984: A marine ichthyofauna from the Miocene of Panama, and the Tertiary Caribbean faunal province. Journal of Vertebrate Paleontology, 42: 172-186

Cirbusova V.; Badonic T.; Marsala J., 1982: Ultrastructural changes in the dorsal funiculi of the spinal cord during ischemia. Effect of partial ischemia on the dorsal cord funiculi was studied in 14 dogs. Gradation of the changes in correlation to the duration of ischemia and to the time for which the experimental animals survived was observed. After ligation below the o...

Thomas, G.R.; Zeitlin, I.J., 1983: Studies on a kallikrein-like amidase in inflammation produced by histamine, carrageenan or Freund's complete adjuvant. Advances in Experimental Medicine and Biology 156: 789-794

Loseto F.; Alessio E.; Mestroni G.; Lacidogna G.; Nassi A.; Giordano D.; Coluccia M., 1991: Interaction of ruthenium chloride dimethylsulphoxide 4 isomers with dna. A wide series of restriction enzymes with a range of specificities was used to investigate the interaction with DNA of Ru(II)-DMSO complexes with anticancer activity. While cis-RuCl2 (DMSO)4 was almost completely inactive, treatment of pBR 322 DNA...

Palma, P.; Khodaei, I., 2015: Dichotomy of rhinoplasty practice: from the conference floor to the operating room. Advancements in surgical techniques and improvements in clinical practice inevitably lag behind scientific progress and peer-led opinion. The rapid rise and fall in the popularity of rhinoplasty techniques makes scientific evidence-gathering and e...

Wilson, A.B.; McHugh, S.M.; Deighton, J.; Ewan, P.W.; Lachmann, P.J., 1993: A competitive inhibition ELISA for the quantification of human interferon-gamma. A competitive enzyme immunoassay has been developed for the measurement of human interferon-gamma (IFN-gamma) in cell culture supernatants. The assay is based on the dose-dependent inhibitory effect of liquid phase IFN-gamma on the binding of a sp...

Owens, J.C., 1966: Survey discovers what is wrong with hospitals' emergency service. Modern Hospital 106(1): 82-85

Mohr, Erna, 1939: Die Jugendfarbung der Brandmaus und die Aalstrich-Frage. The "Aalstrich" is a middorsal line conspicuously darker or lighter than the rest of the pelage. It is seen in the young of the field-mouse and is a not uncommon occurrence in perissodactyls, as the horse, ass, and zebra, but is rare in...

Rao, P.H.; Nandula, S.V.; Murty, V.V., 2008: Molecular cytogenetic applications in analysis of the cancer genome. Cancer cells exhibit nonrandom and complex chromosome abnormalities. The role of genomic changes in cancer is well established. However, the identification of complex and cryptic chromosomal changes is beyond the resolution of conventional banding...