+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Characterization of chicken Nf2/merlin indicates regulatory roles in cell proliferation and migration

, : Characterization of chicken Nf2/merlin indicates regulatory roles in cell proliferation and migration. Developmental Dynamics 229(3): 541-554

The neurofibromatosis 2 (NF2) tumor suppressor protein merlin, or schwannomin, functions as a negative growth regulator such that inactivating mutations in Nf2 predispose humans to tumors. In addition, merlin has a critical role during embryonic development. Nf2-deficient mice die early during embryogenesis, with defects in gastrulation and extraembryonic tissues. To investigate the function of Nf2/merlin during embryonic development, we first identified the homologous Nf2 gene in chicken (cNf2) and examined the distribution of chicken merlin (c-merlin) during myogenesis. cNf2 encoded a full-length mRNA of 1,770 nucleotides and a protein of 589 residues. C-merlin shared high sequence homology and common protein motifs with vertebrate and Drosophila merlins. In addition, cNF2 functions as a negative growth regulator similar to human and Drosophila merlin in vitro. In vivo, c-merlin was expressed diffusely in the forming dermomyotome but down-regulated in migratory muscle precursors in the forelimb. As muscle formed in the limb, c-merlin expression was up-regulated. As an initial examination of c-merlin function during myogenesis, c-merlin was ectopically expressed in muscle precursors and the effects on muscle development were examined. We show that ectopic merlin expression reduces the proliferation of muscle precursors as well as their ability to migrate effectively in limb mesoderm. Collectively, these results demonstrate that c-merlin is developmentally regulated in migrating and differentiating myogenic cells, where it functions as a negative regulator of both muscle growth and motility.


PMID: 14991710

DOI: 10.1002/dvdy.20002

Other references

Schorr, G., 1927: Demands of thanatologie on modern cadaver research methods. The study of the organism as a whole, with special reference to organs and systems of organs, is described with reference to autopsy technic. A scheme for autopsy procedure is given in detail.

Struthers A.D.; Anderson J.V.; Payne N.; Causon R.C.; Slater J.D.H.; Bloom S.R., 1986: The effect of atrial natriuretic peptide on plasma renin activity plasma aldosterone and urinary dopamine in man. The acute natriuretic effect of human atrial natriuretic peptide (ANP) has been well described in man. We have now studied possible hormonal mediators of this effect. We studied six healthy volunteers on two occasions when they received either an...

Forsyth J.C.; Becker C.E.; Osterloh J.; Benowitz N.L.; Rumack B.H.; Hall A.H., 1992: Hydroxocobalamin as a cyanide antidote safety efficacy and pharmacokinetics in heavy smokers. Veterinary & Human Toxicology 34(4): 338

Mamaeva, N.V., 1975: Planktonic infusoria from the volga river ussr. In the section of the Volga river between Kalinin and Astrakhan [Russian SFSR, USSR] 80 Infusoria species were found, 9 of which [Tintinnidium fluiatile, Codonella cratera, Strobilidium velox, Stokesia vernalis, Strombidium viride, Mesodinium pule...

Abjalimov, I.R.; Zinovyeva, M.V.; Nikolaev, L.G.; Kopantzeva, M.R.; Kopantzev, E.P.; Sverdlov, E.D., 2017: Expression of transcription factor genes in cell lines corresponding to different stages of pancreatic cancer progression. The expression level of six transcription factor genes and the content of their protein products in five pancreatic cancer cell lines with parallel control of expression of three marker genes reflecting epithelial or mesenchymal state of cells was...

D.Becker, R., 1966: Apropos of the surgical treatment of Dupuytren's contracture. Acta Chirurgica Belgica 65(9): 935-948

Guy, N.C.; Clarke, J.T.R.; Spence, M.W.; Cook, H.W., 1983: Stimulation of neutral magnesium stimulated sphingomyelinase ec activity in the neuro hypophysis of the rat by hypertonic saline ingestion. Neutral, Mg-stimulated sphingomyelinase and acid sphingomyelinase activities in the neurohypophysis, adenohypophysis and cerebrum of rats given 2.25% NaCl drinking water for 7 or 14 days were compared with the same enzyme activities in the tissues...

Giacometti G.; Turolla A.; Boni R., 1970: Enthalpy of helix coil transitions from heats of solution part 2 poly epsilon carbobenzoxy l lysine and co polymers with l phenyl alanine. Biopolymers 9(8): 979-985

Hosoda, K.; Imoto, Y.; Jobu, J.; Noguchi, M., 1990: Quality evaluation of oriental medicines. I. Quantitative analysis of marker substances in the ethical extract preparation of Hangekouboku-to. For the purpose of evaluating a Kampo preparation, Hangekouboku-to, the contents of 6-gingerol (Gi), guanosine (Gu) and rosmarinic acid (R) in twelve kinds of ethical extract preparations were compared with the self-made decoctions. Little differe...

Furuta, T.; Fujita, M.; Machii, K.; Kobayashi, K.; Kojima, S.; Ueda, K., 1993: Fatal spontaneous pneumocystosis in nude rats. Spontaneously generated fatal pneumocystosis was found in a congenitally athymic nude (rnu/rnu) rat colony. Severe pulmonary pneumocystosis was seen in rnu/rnu rats, and 10(7) to 10(8) cysts per lung were detected. No histologic changes were seen...