geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Characterization of cytosolic glutathione S-transferases in juvenile Chinook salmon (Oncorhynchus tshawytscha)


, : Characterization of cytosolic glutathione S-transferases in juvenile Chinook salmon (Oncorhynchus tshawytscha). Aquatic Toxicology 73(3): 221-229

Four cytosolic glutathione S-transferase (GST) classes were isolated and characterized from juvenile winter run Chinook salmon (Oncorhynchus tshawytscha) liver. Two techniques were used: (1) gel electrophoresis/immunoblotting against a polyclonal striped bass GST antibody and (2) high-pressure liquid chromatography (HPLC). Nanospray liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to elucidate peptide sequences and the proteins were identified as [pi], [theta], [mu] and [alpha], by searching against the NCBI non-redundant database (nrDB). Catalytic activity of the cytosolic GSTs towards 1-chloro-2,4-dinitrobenzene (CDNB) and ethacrynic acid (ETHA) were determined to be 0.3 [plus or minus] 0.05 U/mg cytosolic protein and 0.06 [plus or minus] 0.02 U/mg cytosolic protein, respectively.

US$19.90

PMID: 15935862

DOI: 10.1016/j.aquatox.2005.03.012


Other references

Gøtzsche, P.C., 2006: COX-2 inhibitors and other non-steroidal anti-inflammatory agents--what is their future?. The story of the development, approval, marketing and use of COX-2 inhibitors is one of failures at all levels. Publications in top medical journals essential for marketing were seriously flawed; many cases of thromboses in trials with rofecoxib w...

Aliev M.A.; Khusainova S.K.; Zhuraev S.S.; Seitkalieva S.M., 1990: Role of esophagoscintigraphy in the determination of the treatment tactics for postburn cicatricial esophageal stenosis. Meditsinskaya Radiologiya 35(9): 34

Radler, F., 1965: The main constituents of the surface waxes of varieties and species of the genus Vitis. Cuticular waxes from fresh grapes and leaves of Vitis vinifera, V. labrusca, V. rupestris, several Vitis hybrids and Parthenocissus quinquefolia were extracted with cold chloroform. The main constituent is oleanolic acid, present in the surface wa...

Zhang Yan; Singh, M.B.; Swoboda, I.; Bhalla, P.L., 2005: Agrobacterium-mediated transformation and generation of male sterile lines of Australian canola. An efficient protocol for Agrobacterium-mediated transformation of Australian commercial canola cultivars using seedling explants is described. Seedling explants provide flexibility and reduction in labour and maintenance costs of explant sources....

Brat T., 1971: Depo provera in prolonged action contraception. Schweizerische Zeitschrift fuer Gynaekologie und Geburtshilfe 2(3): 179-190

Walker, A.R.; Latif, A.A.; Morzaria, S.P.; Jongejan, F., 1983: Natural infection rates of Hyalomma anatolicum anatolicum with theileria in Sudan. H. anatolicum anatolicum ticks were collected around cattle pens at 2 locations close to Khartoum [Sudan]. They were assessed for theileria infection by 4 methods. Salivary glands were stained whole with methyl green pyronin and examined for paras...

Halek, C., 1990: Psychodynamic thinking in nursing practice. Nursing Standard 1987) 4(17): 29-31

Van Brunt J., 1986: Drug export bringing it all back home. Bio-Technology (New York) 4(3): 178

Chwilczynski, M., 1972: Deep vessel phlebography of the udder. Isolated udders from 5 Polish Black-and-White Lowland cows aged 3-9 yr were injected through the external pudendal vein with a saturated KI solution, and radiograms were taken from lateral and dorso-ventral aspects. Radiograms and drawings based o...

Lopatta F.; Vecsei P.; Taeuber U.; Haack D., 1986: Pharmacokinetics of fluocortolone during long term treatment of patients with rheumatic and hematological diseases. The pharmacokinetics of fluocortolone (Ultralan oral) and its effect on plasma cortisol levels were investigated during long-term treatment of patients with rheumatic and haematological diseases with different doses of fluocortolone. Fluocortolone...