+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Characterization of flavonoids by aluminum complexation and collisionally activated dissociation

, : Characterization of flavonoids by aluminum complexation and collisionally activated dissociation. Journal of Mass Spectrometry 40(3): 350-363

Aluminum complexes of the type (AlIII (flavonoid - H2)+ are generated by electrospray ionization in order to allow differentiation of isomeric flavonoids by tandem mass spectrometry. The dominant species observed from the aluminum complexation reaction has a 1:2 aluminum(III):flavonoid stoichiometry. Differentiation of 18 flavonoids constituting seven isomeric series was achieved based on the collisionally activated dissociation patterns of the aluminum complexes. Characteristic fragmentation pathways allow identification of the site of glycosylation, the type of saccharide (rutinose versus neohesperidose) and the type of bond between the C-2 and C-3 atoms (thus distinguishing flavanones from flavonols and flavones). Two stable coordination geometries of the aluminum complex of apigenin were identified. The non-planar structure with a plane-angle of nearly 90degree is 25.3 kcal mol-1 more favorable than the planar structure. The conformations of the complexes, which involve multiple interactions between the aglycone and disaccharide portions of the flavonoid with the metal ion, are significantly different for the isomeric flavonoids. Copyright Copyright 2005 John Wiley & Sons, Ltd.


PMID: 15674859

DOI: 10.1002/jms.793

Other references

Andersen, T., 1958: Survey of the statistical results of Danish Milk Recording Societies, 1956-57. During 1956-57, 868, 393 cows, or 60% of Denmark's total dairy herd, were milk recorded. Their average milk yield was 4, 038 kg. and the average butterfat content in the milk 4.34%. The Red Danish, Black and White Danish, Jersey and Shorthorn...

Varnum, B.C.; Lim, R.W.; Herschman, H.R., 1989: Characterization of TIS7, a gene induced in Swiss 3T3 cells by the tumor promoter tetradecanoyl phorbol acetate. We report here the nucleic acid sequence and deduced amino acid sequence of a cDNA for TIS7, a gene induced by mitogens in 3T3 cells and by nerve growth factor in PC12 pheochromocytoma cells. A fragment of the TIS7 gene has previously been cloned...

Schaper, C.; Zhu, Y.; Kouklei, M.; Culmsee, C.; Krieglstein, J., 2000: Stimulation of 5-HT1A receptors reduces apoptosis after transient forebrain ischemia in the rat. It has recently been shown that 5-HT(1A) receptor stimulation reduced the infarct volume after occlusion of the middle cerebral artery in rats. Since there is increasing evidence that apoptosis is involved in neurodegenerative diseases and stroke,...

Pratt, 2000: Lifestyle Physical Activity. Current Treatment Options in Cardiovascular Medicine 1(3): 203-208

Qiu, X.; Daly, M.J.; Vasilenko, A.; Omelchenko, M.V.; Gaidamakova, E.K.; Wu, L.; Zhou, J.; Sundin, G.W.; Tiedje, J.M., 2006: Transcriptome analysis applied to survival of Shewanella oneidensis MR-1 exposed to ionizing radiation. The ionizing radiation (IR) dose that yields 20% survival (D-20) of Shewanella oneidensis MR-1 is lower by factors of 20 and 200 than those for Escherichia coli and Deinococcus radiodurans, respectively. Transcriptome analysis was used to identify...

Naumann, U.Kolyvanos.; Käser, L.; Vetter, W., 2006: Arterial hypertension 3: Hypertensive crisis: hypertensive emergency and hypertensive urgency. Praxis 95(25-26): 1011-8; Quiz 1019-20

Kim, L.G.; Lee, D.S.; Hahn, W.S., 1990: Development of a farm management analysis programme for a personal computer. A program suitable for a personal computer which uses spreadsheet software to provide a farm management analysis is presented. The output provides information on management expenses, labour input and farm management and expenditure and provides ba...

Ye, P.; Li, J.; Wang, S.; Xie, A.; Sun, W.; Xia, J., 2012: Eicosapentaenoic acid disrupts the balance between Tregs and IL-17+ T cells through PPARγ nuclear receptor activation and protects cardiac allografts. Eicosapentaenoic acid (EPA) is one of n-3 polyunsaturated fatty acids that possesses a wide array of anti-inflammatory effects but its effects, on transplantation in general and on Tregs and IL-17(+) T cells in particular, are not well studied. We...

Koirtyohann S.R.; Sievers H.; Pickett E.E., 1972: The electrically heated furnace in atomic absorption. Proceedings of University of Missouri's Annual Conference on Trace Substances in Environmental Health 5: 463-471

Garton, G.A.; Oxford, A.E., 1955: The nature of bacterial lipids in the rumen of hay-fed sheep. Two wether sheep with rumen fistulae were fed on meadow hay, with access to water and a mineral lick. Samples of rumen contents were taken every second day and centrifuged to give a fraction consisting mainly of bacteria. The samples were combined...