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Cloning, expression of recA gene from Deinococcus radiodurans and the effect of radioresistance of recA defective Escherichia coli


, : Cloning, expression of recA gene from Deinococcus radiodurans and the effect of radioresistance of recA defective Escherichia coli. Weishengwu Xuebao 44(1): 41-44, February

The recA gene of D. radiodurans was cloned into expression vector pET15b and a recombinant fusion RecA protein was overexpressed in E. coli HMS (DE3) by IPTG induction. On the other hand, we have succeeded in the normal expression of the cloned recA gene of D. radiodurans in E. coli via a shuttle plasmid pRADZ3 under the control of GroES promoter without induction of IPTG. The expression of recA gene of D. radiodurans in E. coli TG2 was able to completely complement its defective radioresistance, caused by the intrinsic recA deficiency, following exposure to ionizing radiation.

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