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Comparison of three different staining methods for the assessment of epididymal red deer sperm morphometry by computerized analysis with ISAS (R)

, : Comparison of three different staining methods for the assessment of epididymal red deer sperm morphometry by computerized analysis with ISAS (R). Theriogenology 64(5): 1236-1243

When collection of ejaculated sperm samples is not possible, as is the case with wild species, the epididymides of sacrificed wild males become the only possible source of spermatozoa. Mature cauda epididymal spermatozoa display characteristics similar to those of ejaculated sperm cells. The present work proposes a sperm staining technique suitable for the morphometric evaluation of red deer epididymal sperm using a new computerized system. Epididymides from wild animals were extracted no later than 2 h post mortem. After epididymal sectioning, sperm samples were collected, cooled to and equilibrated at 5 degrees C, and frozen in liquid nitrogen. Before staining, sperm samples were thawed for 20 s at 37 degrees C, and used for the preparation of slides. Three different sperm stains were tested: Hemacolor, Diff-Quik, and Harris' Hematoxylin. Morphometric analyses of sperm samples were performed using the morphologic module of the ISAS (R). Two hundred spermatozoa per sample and stain were captured at random and analyzed. Sperm morphometric values were significantly affected by the staining technique used. Moreover, significant differences were observed between animals. In our study, Diff-Quik could be considered to be the best sperm staining method, as it provided the highest percentage of well automatically analyzed cells by the ISAS (R), and discriminates better between animals. This sperm staining technique also proved to be a useful method for characterizing and discriminating between sperm samples of different animals. (c) 2005 Elsevier Inc. All rights reserved.


DOI: 10.1016/j.theriogenology.2005.02.018

Other references

Neeser, J.R.; Schweizer, T.F., 1984: A quantitative determination by capillary gas-liquid chromatography of neutral and amino sugars (as O-methyloxime acetates), and a study on hydrolytic conditions for glycoproteins and polysaccharides in order to increase sugar recoveries. Complete gas-liquid chromatographic separation of O-methyloxime acetates (syn and anti isomers) prepared from eight neutral sugars, three hexosamines, and muramic acid has been obtained, using a fused-silica Carbowax 20M capillary column. A single...

E.K.ordagui, L.K.; Nada, A.H.; Molokhia, A.M.; Hammouda, Y.E., 1996: Process and material optimization and bioavailability evaluation of salbutamol tablets. Pharmaceutical Research (New York) 13(9 SUPPL ): S207

Ottesen, B.S., 1998: The Endometriosis Association--a new patients' association. Ugeskrift for Laeger 160(3): 296-296

Schiwara, H.W., 1993: Mercury sensitization with amalgam fillings. Deutsche Medizinische Wochenschrift 118(48): 1784-1785

Murohashi, T.; Yoshida, K., 1968: Biology of the mycobacterioses. Biological significance of acid-fastness of mycobacteria. Annals of the New York Academy of Sciences 154(1): 58-67

Bridges, S.; Higginbotham, C.M.kinion, J.; Hodges, J., 1995: Fuzzy descriptors of time-varying data: theory and application. AI applications(2): 1-14

Schiopu M.; Tudosie M.; Popoviciu L.; Roman V.; Pascu I., 1983: Computerized electro encephalographic map a noninvasive method for the dynamic study of the electrographic anomalies in cerebro vascular diseases. Revue Roumaine de Medecine Neurologie et Psychiatrie 21(3): 194-196

Meusel H., 1968: Growth form and distribution of atractylis humilis d and related species. Collectanea Botanica (Barcelona) 7(2): 799-816

Summers, J.J.; Sommer, J.A.; Sharp, C.A.; Levey, A.; Murray, K.D., 1982: The coding of constrained and preselected movement distance: same-limb versus switched-limb reproduction. Acta Psychologica 51(1): 75-89

Ketterer D.M.; Jackson Cook C.K.; Howard Peebles P.N.; Casey J.; Heisler K.L.; Daugherty E.A.; Giles H.R., 1991: Triple nor variants clinical comparisons. American Journal of Human Genetics 49(4 SUPPL): 221