geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Complement activation by core-shell poly(isobutylcyanoacrylate)-polysaccharide nanoparticles: influences of surface morphology, length, and type of polysaccharide


, : Complement activation by core-shell poly(isobutylcyanoacrylate)-polysaccharide nanoparticles: influences of surface morphology, length, and type of polysaccharide. Pharmaceutical Research 23(6): 1313-1323

Purpose. Biodistribution of intravenously administered nanoparticles depends on opsonization. The aim of this study was the evaluation of complement activation induced by nanoparticles coated with different polysaccharides. Influences of size and configuration of dextran, dextran sulfate, or chitosan bound onto nanoparticles were investigated.Method. Core-shell nanoparticles were prepared by redox radical or anionic polymerization of isobutylcyanoacrylate in the presence of polysaccharides. Conversion of C3 into C3b in serum incubated with nanoparticles was evaluated.Results. Cleavage of C3 increased with size of dextran bound in "loops" configuration, whereas it decreased when dextran was bound in "brush." It was explained by an increasing steric repulsive effect of the brush, inducing poor accessibility to OH groups. The same trend was observed for chitosan-coated nanoparticles. Nanoparticles coated with a brush of chitosan activated the complement system lesser than nanoparticles coated with a brush of dextran. This was explained by an improved repelling effect. Dextran-sulfate-coated nanoparticles induced a low cleavage of C3 whereas it strongly enhanced protein adsorption.Conclusion. Complement activation was highly sensitive to surface features of the nanoparticles. Type of polysaccharide, configuration on the surface, and accessibility to reactive functions along chains are critical parameters for complement activation.

US$19.90

PMID: 16715369

DOI: 10.1007/s11095-006-0069-0


Other references

Rocca, B.; Maggiano, N.; Habib, A.; Lauriola, L.; Landolfi, R.; Ranelletti, F.O., 1999: Constitutive and selective expression of cyclooxygenase-2 in medullary epithelial cells of human thymus. Blood 94(10 SUPPL 1 PART 2): 57b, Nov 15

Nutman T.B.; Miller K.D.; Mulligan M.; Reinhart G.N.; Currie B.; Steel C.; Ottesen E.A., 1988: Diethylcarbamazine provides effective prophylaxis for human loiasis results of a double blinded study. Clinical Research 36(3): 465A

Williams, R.; Lim, J.E.; Harr, B.; Wing, C.; Walters, R.; Distler, M.G.; Teschke, M.; Wu, C.; Wiltshire, T.; Su, A.I.; Sokoloff, G.; Tarantino, L.M.; Borevitz, J.O.; Palmer, A.A., 2009: A common and unstable copy number variant is associated with differences in Glo1 expression and anxiety-like behavior. Glyoxalase 1 (Glo1) has been implicated in anxiety-like behavior in mice and in multiple psychiatric diseases in humans. We used mouse Affymetrix exon arrays to detect copy number variants (CNV) among inbred mouse strains and thereby identified a...

de Lisle, HF., 1976: Coral snake mimicry. Herpetology, 82: 18-24

Fermin, C.D.; Lovett, A.E.; Igarashi, M.; Dunner, K., 1990: Immunohistochemistry and histochemistry of the inner ear gelatinous membranes and statoconia of the chick (Gallus domesticus). This study was undertaken to investigate whether the gelatinous membranes (GMs) of the chick (Gallus domesticus) inner ear contain glycoproteins and proteoglycans, as is the case in mammals, and whether or not the relative concentrations of glycop...

Burda, D., 1994: W.Va. gov unveils reform package. Modern Healthcare 24(5): 16-16

Wolf, B.S., 1962: Radiological notes: case No. 159. Journal of the Mount Sinai Hospital, New York 29: 99-102

Welsh, J.P.; Bonomo, J.; Swartz, J.R., 2011: Localization of BiP to translating ribosomes increases soluble accumulation of secreted eukaryotic proteins in an Escherichia coli cell-free system. The endoplasmic reticulum (ER) resident Hsp70 chaperone, BiP, docks to the Sec translocon and interacts co-translationally with polypeptides entering the ER to encourage proper folding. In order to recreate this interaction in Escherichia coli cel...

Lopez; Hirsa, 2000: Surfactant-Influenced Gas-Liquid Interfaces: Nonlinear Equation of State and Finite Surface Viscosities. A canonical flow geometry was utilized for a fundamental study of the coupling between bulk flow and a Newtonian gas-liquid interface in the presence of an insoluble surfactant. We develop a Navier-Stokes numerical model of the flow in the deep-ch...

Byhahn, C.; Strouhal, U.; Westphal, K., 2000: Exposure of anesthetists to sevoflurane and nitrous oxide during inhalation anesthesia induction in pediatric anesthesia. Inhalational mask induction with nitrous oxide and sevoflurane in young children is an appropriate alternative to intravenous induction and is considered safe and of rapid onset. Disadvantages of this technique are environmental pollution and occu...