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Correlation between dose of ultraviolet B irradiation and apoptotic phase of HaCaT cells


, : Correlation between dose of ultraviolet B irradiation and apoptotic phase of HaCaT cells. Zhonghua Pifuke Zazhi 38(7): 435-438

Objective To elucidate the correlation between the dose of ultraviolet B (UVB) irradiation and apoptotic phase of keratinocytes (HaCaT cells). Methods Cultured immortalized human keratinocyte cell line, HaCaT cells, was irradiated with ultraviolet B (UVB) in doses of 20, 40, 60, 80, 100 and 120 mJ/cm(2). Cellular viability, cell cycles and apoptosis were simultaneously detected at 2, 12, 24, 48 and 72 h after irradiation.

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