+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Covalent cross-linking of the NC1 domain of collagen type IX to collagen type II in cartilage

, : Covalent cross-linking of the NC1 domain of collagen type IX to collagen type II in cartilage. Journal of Biological Chemistry 279(4): 2568-2574

From a study to understand the mechanism of covalent interaction between collagen types II and IX, we present experimental evidence for a previously unrecognized molecular site of cross-linking. The location relative to previously defined cross-linking sites predicts a specific manner of interaction and folding of collagen IX on the surface of nascent collagen II fibrils. The initial evidence came from Western blot analysis of type IX collagen extracted by pepsin from fetal human cartilage, which showed a molecular species that had properties indicating an adduct between the alpha1(II) chain and the C-terminal domain (COL1) of type IX collagen. A similar component was isolated from bovine cartilage in sufficient quantity to confirm this identity by N-terminal sequence analysis. Using an antibody that recognized the putative cross-linking sequence at the C terminus of the alpha1(IX) chain, cross-linked peptides were isolated by immunoaffinity chromatography from proteolytic digests of human cartilage collagen. They were characterized by immunochemistry, N-terminal sequence analysis, and mass spectrometry. The results establish a link between a lysine near the C terminus (in the NC1 domain) of alpha1(IX) and the known cross-linking lysine at residue 930 of the alpha1(II) triple helix. This cross-link is speculated to form early in the process of interaction between collagen IX molecules and collagen II polymers. A model of molecular folding and further cross-linking is predicted that can spatially accommodate the formation of all six known cross-linking interactions to the collagen IX molecule on a fibril surface. Of particular biological significance, this model can accommodate potential interfibrillar as well as intrafibrillar links between the collagen IX molecules themselves, so providing a mechanism whereby collagen IX could stabilize a collagen fibril network.


PMID: 14602708

DOI: 10.1074/jbc.M311653200

Other references

Randall, C.N.; Strasburger, D.; Prozonic, J.; Morris, S.N.; Winkie, A.D.; Parker, G.R.; Cheng, D.; Fennell, E.M.; Lanham, I.; Vakil, N.; Huang, J.; Cathcart, H.; Huang, R.; Poduslo, S.E., 2008: Cluster analysis of risk factor genetic polymorphisms in Alzheimer's disease. Multiple genetic variants may contribute to the risk of developing Alzheimer's disease. We have analyzed polymorphisms in 9 genes to determine whether particular combinations would contribute to this risk. The genes were APOE, LDLr, CST3, CTS...

Saraceni Richards, C.; Jacobson, G.R., 1993: Site-directed mutagenesis of a conserved glutamate residue which may be involved in substrate binding by the mannitol permease of Escherichia coli. FASEB Journal 7(7): A1141

Ganten, M-Katharina.; Weber, T.F.; von Tengg-Kobligk, H.; Böckler, D.; Stiller, W.; Geisbüsch, P.; Kauffmann, Günter.W.; Delorme, S.; Bock, M.; Kauczor, H-Ulrich., 2008: Motion characterization of aortic wall and intimal flap by ECG-gated CT in patients with chronic B-dissection. To evaluate whether dynamic computed tomography (CT)-imaging can provide functional vessel information in patients with chronic aortic dissection type Stanford-B (ADB). In 32 patients, ECG-gated CT-angiography images were obtained. Cross-sectional...

Lekadir, K.; Hoogendoorn, Cé.; Pereanez, M.; Albà, Xènia.; Pashaei, A.; Frangi, A.F., 2014: Statistical personalization of ventricular fiber orientation using shape predictors. This paper presents a predictive framework for the statistical personalization of ventricular fibers. To this end, the relationship between subject-specific geometry of the left (LV) and right ventricles (RV) and fiber orientation is learned stati...

Snow, E.W., 1963: Use of stanazolol in severe debility. Northwest Medicine 62: 194-194

Anonymous, 1998: Annual report , No. 49, 1988. This report contains over 150 short papers on research conducted at the National Institute of Genetics (Japan) during 1998. The papers cover work on bacteria, viruses, yeasts, crop plants, nematodes, insects, laboratory mammals and man. The topics...

Oliver, S., 1996: Midwives' attitudes devalue nurses. NlmCategory="UNASSIGNED">I continue to be dismayed at midwives' attitudes to their nursing colleagues and their suggestions that they deserve a pay rise comparable with junior doctors.

Dietze, Kathrin, 1997: Skull morphology and intra-specific variation of Paramblypterus duvernoyi and its bearing on the systematics of paramblyptereded fishes. Journal of Vertebrate Paleontology 17(3 SUPPL ): 43A

Simão, Iês.; Cordeiro, A.Isabel.; Borrego, Lís.Miguel.; Martins, C.; Neves, Cção.; Neves, Jão.Farela., 2013: Monoclonal IgG kappa gammopathy previous to hematopoietic stem cell transplantation in an infant with severe combined immunodeficiency. Clinical Immunology 145(2): 133-135

Savov, I.; Ichev, K., 1987: An electron microscopic study on pneumocytes of ii type in embryonal lung of a rat. The aim of this study was to examine morphology of pneumocytes of II type in embryos of a white rat at various age i.e. with a different degree of immaturity of pneumocytes respectively their surfactant system. Differences in the morphology of mul...