geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

DNA adduct formation and physiological effects from crude oil distillate and its derived base oil in isolated, perfused rat liver


, : DNA adduct formation and physiological effects from crude oil distillate and its derived base oil in isolated, perfused rat liver. Archives of Toxicology 78(2): 114-121

A distillate, D431, originating from Venezuelan heavy crude oil and a severely hydro-treated base oil, BO100, derived from this distillate, were tested for DNA adduct formation capacities and overall impact on liver functions. D431 had earlier showed DNA adduct formation in vitro but not in vivo in the rat. In this study, isolated rat liver perfusions were performed to elucidate whether the lack of DNA adducts in vivo was because of lack of uptake or metabolism. The oils were extracted with dimethyl sulfoxide and the extracts added to the perfusion system. Bile production, lactate metabolism and perfusate flow through the organ, which are parameters that reveal the condition of the liver, were continuously monitored. Samples of liver were collected once every hour during perfusion for DNA adduct analysis with (32)P-HPLC. The results for the distillate D431 showed that the production of bile and metabolism of lactate decreased while DNA adduct formation increased with time. The DNA adduct pattern formed in the D431-treated livers was similar to that found earlier in in vitro studies performed on calf thymus DNA (CT-DNA). In the case of DNA adduct formation, virtually no difference with dose was seen, suggesting that perhaps a point of saturation of, for instance, enzymatic systems was reached. The results for base oil BO100 showed no significant difference regarding bile production, lactate metabolism and DNA adduct formation when compared with the control, indicating no toxic or genotoxic activity.

US$19.90

PMID: 14647976

DOI: 10.1007/s00204-003-0489-0


Other references

Lanser M.E.; Saba T.M., 1981: Opsonic fibronectin is necessary for optimal serum mediated phagocytosis of staphylococcus aureus by human and rat neutrophils. Circulatory Shock 8(2): 209

Raineri, Valter, 1995: On some interesting Geometrid moths collected in Greece. The Author reports some data on Geometridae from Greece.

Takemoto, T.; Tada, M.; Dittler, H.J.; Rösch, T.; Siewert, J.R., 1993: Impact of staging on treatment of gastric carcinoma. Endoscopy 25(1): 46-50

Roberson, J.R.; Warnick, L.; Swecker, W.S.; Whittier, W.D.; Blodgett, D.J.; Robertson, J.L., 1997: Acute respiratory distress syndrome in adult beef cattle fed peanut-vine hay. In the autumn of 1993 an outbreak of acute respiratory distress syndrome (ARDS) occurred in southeastern Virginia in mature cattle being fed peanut-vine hay. The incidence of the disease was highest in November and December and the case fatality r...

Cornish, G.A., 1998: Pressurized irrigation technologies for smallholders in developing countries - a review. The findings of a study of factors influencing the uptake of pressurized irrigation technologies by smallholders in developing countries are presented. The paper reviews the physical and technical characteristics that determine their suitability f...

Almeida, P.R.L.; Mattos, A.A.; Both, C.T.; Peixoto, M.F.; Veronese, F.; Flor, C.; Lima, A.A., 1994: Histological aspects of virus C hepatopathy in blood donors and hemodialysis patients A preliminary and comparative analysis. Hepatology 20(4 PART 2): 327A

Stehl, M.J.Lutz.; Kazak, A.E.; Hwang, W-Ting.; Pai, A.L.H.; Reilly, A.F.; Douglas, S.D., 2008: Innate immune markers in mothers and fathers of children newly diagnosed with cancer. The diagnosis of a life-threatening illness in a child is one of the most stressful events imaginable for parents and is associated with increased anxiety and distress. Despite associations between stress and immune function in animal and human mo...

Holmberg, H., 1992: Butterflies of Lappland 1991.. Baptria (Helsinki): 174: 89-95

Rastsvetayev L.M., 1980: The main features of the structural pattern of Earth's surface and its dynamic interpretation. Trudy Geologicheskiy Institut, Akademiya Nauk SSSR: Pages 145-197. 1980.

Charng, M.J.; Frenkel, P.A.; Lin, Q.; Olson, E.N.; Overbeek, P.A.; Schneider, M.D., 1997: Constitutive signaling by ALK5 disrupts normal looping morphogenesis in transgenic mice. Circulation 96(8 SUPPL ): I512, 10/21/97