+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

DNA nucleic acid sequence-based amplification-based genotyping for polymorphism analysis

, : DNA nucleic acid sequence-based amplification-based genotyping for polymorphism analysis. Biotechniques 37(4): 680-2, 684, 686

Nucleic acid sequence-based amplification (NASBA) is a sensitive isothermal transcription-based amplification method known to be a suitable tool for RNA research. We demonstrate that NASBA technology can be applied to single nucleotide polymorphism (SNP) analysis using human genomic DNA as a template. Combination of DNA NASBA with multiplex hybridization of specific molecular beacons makes it possible to unambiguously discriminate the presence of the SNP of interest.


PMID: 15517981

Other references

Szawlowski, P.W.; Al-Safi, S.A.; Dooley, T.; Maddocks, J.L., 1985: Azathioprine suppresses the mixed lymphocyte reaction of patients with Lesch-Nyhan syndrome. The mixed lymphocyte reaction of Lesch-Nyhan patients (HGPRT deficient) was used to study the immunosuppressive effects of azathioprine and 6-mercaptopurine (6-MP). Mitogen stimulated lymphocytes of these patients are highly resistant to azathiopr...

Kreshkov, A.P.; Chivikova, A.N.; Zagorovskaya, A.A., 1967: A new rapid method for determining free amorphous silicon dioxide in clays. The SiO2 is dissolved by adding glycerine to the sample, and the resulting solution is titrated with Ba(OH)2 in aqueous glycerine, with phenolphthalein or alizarin yellow as indicator.

Zambrano-Zaragoza, Jé.Francisco.; Durán-Avelar,ús.; Messina-Robles, M.; Vibanco-Pérez, N., 2012: Characterization of the humoral immune response against Gnathostoma binucleatum in patients clinically diagnosed with gnathostomiasis. Gnathostomiasis is an emerging systemic parasitic disease acquired by consuming raw or uncooked fresh-water fish infected with the advanced third-stage larvae of Gnathostoma spp. This disease is endemic to the Pacific region of Mexico, and one of...

Varela, A.S.; Stallknecht, D.E.; Yabsley, M.J.; Moore, V.A.; Davidson, W.R.; Little, S.E., 2004: Experimental infection of white-tailed deer (Odocoileus virginianus) with Ehrlichia chaffeensis by different inoculation routes. The infection dynamics of the tick-transmitted organism Ehrlichia chaffeensis were investigated in white-tailed deer (Odocoileus virginianus) using different routes of inoculation. Six deer were each inoculated with 5.4 x 10(6) DH82 cells infected...

Hartl, W., 1967: Modern concepts of the pathogenesis of primary chronic polyarthritis. Die Medizinische Welt 1: 15-18

Setta, K.; Oida, K.; Ogawa, K., 1966: Paget's disease of the nipple in a young pregnant woman. Nihon Geka Hokan. Archiv für Japanische Chirurgie 35(2): 432-435

Topalcengiz, Z.; Strawn, L.K.; Danyluk, M.D., 2017: Microbial quality of agricultural water in Central Florida. The microbial quality of water that comes into the edible portion of produce is believed to directly relate to the safety of produce, and metrics describing indicator organisms are commonly used to ensure safety. The US FDA Produce Safety Rule (PS...

Seidler, R.D.; Gluskin, B.S.; Greeley, B., 2016: Right Prefrontal Cortex Transcranial Direct Current Stimulation Enhances Multi-Day Savings in Sensorimotor Adaptation. NlmCategory="UNASSIGNED">We have previously reported that visuospatial working memory performance and magnitude of activation in the right dorsolateral prefrontal cortex predict the rate of visuomotor adaptation. Recent behavioral stu...

Temming, P.; Viehmann, A.; Arendt, M.; Eisele, L.; Spix, C.; Bornfeld, N.; Sauerwein, W.; Jöckel, K-Heinz.; Lohmann, D.R., 2015: Pediatric second primary malignancies after retinoblastoma treatment. Children with retinoblastoma carry a high risk to develop second primary malignancies in childhood and adolescence. This study characterizes the type of pediatric second primary malignancies after retinoblastoma treatment and investigates the impa...

Nelson, O.Lynne.; Robbins, C.T., 2016: Cardiovascular function in large to small hibernators: bears to ground squirrels. Mammalian hibernation has intrigued scientists due to extreme variations in normal seasonal physiological homeostasis. Numerous species manifest a hibernation phenotype although the characteristics of the hypometabolic state can be quite different...