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Development of white blood cell fragments, during the preparation and storage of platelet concentrates, as measured by using real-time polymerase chain reaction


, : Development of white blood cell fragments, during the preparation and storage of platelet concentrates, as measured by using real-time polymerase chain reaction. Vox Sanguinis 87(4): 250-256

Background and Objectives White blood cell (WBC) fragments may cause human leucocyte antigen (HLA) immunization in recipients. We investigated the occurrence and production of WBC fragments in platelet concentrates (PCs) and plasma units, during storage and filtration, by using real-time polymerase chain reaction (PCR) and flow cytometry. Materials and Methods To study the occurrence of WBC fragments, 'male' WBCs were spiked into double-filtered 'female' PCs in a concentration series of 0.03-100 WBCs/mul (n = 4 per level). To study the production of WBC fragments, 'male' WBCs were spiked into 'female' plasma units to 4 x 109 WBCs/l and stored at room temperature prior to filtration (n = 4 per storage time; t = 0, 24 or 48 h). DNA was measured by both albumin real-time PCR and Y real-time PCR. Intact WBCs were counted by using flow cytometry. The number of WBC fragments was calculated by subtracting cell-free DNA (real-time PCR on supernatant) and intact WBCs (flow cytometry) from the total DNA amount (real-time PCR). Results Spiking of 'male' WBCs into 'female' PCs showed that the Y real-time PCR is linear and has a reproducible quantitative range down to 0.03 WBC/mul, but that the albumin-PCR, in unspiked samples, revealed a total of 6-10 WBC equivalents/mul (eq/mul). After centrifugation, half of this was observed as cell-free DNA in the supernatant, suggesting that the remaining DNA is derived from WBC fragments. The number of intact WBCs, amount of cell-free DNA and number of WBC fragments after filtration increased significantly when filtration was delayed for up to 48 h, from 0.1 WBC/mul, 1.3 WBC eq/mul and 0.6 WBC eq/mul at t = 0 h to 25 WBC/mul, 38 WBC eq/mul and 57 WBC eq/mul at t = 48 h, respectively. Conclusions WBC fragments occur in WBC-reduced PCs and increase when products are stored, prior to filtration, up to levels that are equivalent to the amounts of intact WBCs that induce HLA immunization (i.e. > 5 x 106/unit).

US$19.90

PMID: 15585020

DOI: 10.1111/j.1423-0410.2004.00576.x


Other references

Chen, Z.; Zhang, W.; Ni, L.; Wang, G.; Cao, Y.; Wu, W.; Sun, C.; Yuan, D.; Ni, H.; Wang, Y.; Yang, H., 2015: Spatiotemporal Expression of Poly(rC)-Binding Protein PCBP2 Modulates Schwann Cell Proliferation After Sciatic Nerve Injury. Poly(C)-binding proteins (PCBPs), also known as RNA-binding proteins, interact in a sequence-specific fashion with single-stranded poly(C). It was reported that PCBP2 contributed to gastric cancer proliferation and survival through miR-34a, and kn...

Scambler, A.; Scambler, G.; Ridsdale, L.; Robins, D., 1996: Towards an evaluation of the effectiveness of an epilepsy nurse in primary care. This paper reports on early results of an investigation of the effectiveness of a specialist epilepsy nurse in primary care. Based on before-and-after interviews with sub-samples of adults with epilepsy, these results suggest that not only are exp...

Elveland J.; Ohlson M., 1987: Vegetational history and recent conditions in the 2 northernmost localities for thelypteris palustris in sweden. Thelypteris palustris Schott has a circumpolar distribution. The species reaches its northern limit in north Sweden, where two isolated localities are situated. The vegetational hsitory, with special regard to the occurrence of Thelypteris palustr...

Chornet, E.; Czernik, S., 2002: Harnessing hydrogen. In this issue, Cortright et al. provide evidence that simple biomass-derived molecules can be treated to produce hydrogen with reasonable efficiency. Biomass can produce clean fuels and could be a vital, renewable energy source for tomorrow's...

Seid, B., 1958: Management of seminal vesiculitis. Illinois Medical Journal 113(6): 297-299

Robertson, S.E.; Roca, A.; Alonso, P.; Simoes, E.A.F.; Kartasasmita, C.B.; Olaleye, D.O.; Odaibo, G.N.; Collinson, M.; Venter, M.; Zhu, Y.; Wright, P.F., 2005: Respiratory syncytial virus infection: denominator-based studies in Indonesia, Mozambique, Nigeria and South Africa. To assess the burden of respiratory syncytial virus (RSV)-associated lower respiratory infections (LRI) in children in four developing countries. A WHO protocol for prospective population-based surveillance of acute respiratory infections in child...

Steggles A.W., 1982: Di ethyl stilbestrol induced cyst adenomatous papilloma and uterus hypoplasia in the hamster. Anatomical Record: 64a

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