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Effects of extracellular Ca(2+) influx and intracellular Ca(2+) release on ethanol-induced cytoplasmic Ca(2+) overload in cultured superior cervical ganglion neurons


, : Effects of extracellular Ca(2+) influx and intracellular Ca(2+) release on ethanol-induced cytoplasmic Ca(2+) overload in cultured superior cervical ganglion neurons. Neuroscience Letters 390(2): 98-103

The present research was designed to investigate the interference of Ca2+ homeostasis by ethanol on the primary cultured superior cervical ganglion (SCG) neurons. (1) Using the whole cell patch clamp recording, the amplitudes of voltage-dependent Ca2+ channel (VDCC) currents could be reduced by ethanol in a concentration-dependent manner. Ethanol (100 mM) inhibited about 25% of Ca2+ channel current. However, the activation of Ca2+ channel was not affected by ethanol at those concentrations. (2) The similar extent inhibitions of 100 MM ethanol on the increments of intracellular Ca2+ concentration ([Ca2+](i)) induced by 40 mM KCl and 1 mu M A23187 were also observed in the fluo-3-AM loaded superior cervical ganglia (SCG) via detecting the change of [Ca2+](i) with a laser scanning confocal microscopy. In contrast, the basal [Ca2+](i) was significantly increased by ethanol alone in a concentration-dependent manner. These phenomena were also observed even under Ca2+ free bath solution or the solution added 300 mu M cadmium chloride conditions. Together with above results, our data suggest that ethanol increases basal [Ca2+](i), but it also inhibits the extracellular Ca2+ influx through VDCC and ionophore channel. And the augment of basal [Ca2+](i) induced by ethanol might attribute to the Ca2+ releasing from intracellular Ca2+ pools. (C) 2005 Elsevier Ireland Ltd. All rights reserved.

US$19.90

PMID: 16115728

DOI: 10.1016/j.neulet.2005.08.004


Other references

Richmond, C.A.; Graham, H.M., 1970: Suppression of populations of pink boll-worms with releases of sterilized moths in field cages. In continued studies in Texas on the sterilisation of Pectinophora gossypiella (Saund.) by means of gamma -radiation, some hatching occurred in eggs of which only the male parent had been treated at 25-40 krad, but these eggs gave rise to sterile...

Knychalska-Karwan, Z.; Pawlicki, R.; Jakób-Dolezal, K.; Karwan, T., 1982: Hard teeth tissues in rats with experimental rickets. Examinations in electron scanning microscope and microanalyser. The experiments were carried out on Wistar rats with rickets experimentally induced. The following examinations were made: radiological examinations of the jaws, a teeth analysis in the microanalyser for the distribution of fundamental elements, a...

Peixoto, R.; Chaer, G.M.; Carmo, F.L.; Araújo, F.V.; Paes, J.E.; Volpon, A.; Santiago, G.A.; Rosado, A.S., 2011: Bacterial communities reflect the spatial variation in pollutant levels in Brazilian mangrove sediment. The majority of oil from oceanic oil spills converges on coastal ecosystems such as mangrove forests. A major challenge to mangrove bioremediation is defining the mangrove's pollution levels and measuring its recuperation from pollution. Bioi...

Flaquer, Aònia.; Rospleszcz, S.; Reischl, E.; Zeilinger, S.; Prokisch, H.; Meitinger, T.; Meisinger, C.; Peters, A.; Waldenberger, M.; Grallert, H.; Strauch, K., 2016: Mitochondrial GWA Analysis of Lipid Profile Identifies Genetic Variants to Be Associated with HDL Cholesterol and Triglyceride Levels. It has been suggested that mitochondrial dysfunction has an influence on lipid metabolism. The fact that mitochondrial defects can be accumulated over time as a normal part of aging may explain why cholesterol levels often are altered with age. To...

Archibald, J.M., 2017: Evolution: Protein Import in a Nascent Photosynthetic Organelle. An amoeba named Paulinella harbours 'chromatophores', cyanobacterium-derived photosynthetic bodies that evolved independent of plastids. Proteomics has shown that hundreds of nucleus-encoded proteins are targeted to the chromatophore, re...

Vaillant, F., 1952: Les composantes de la faune hygropetrique des Alpes du Dauphine. Compte Rendu du Congres des Societes Savantes de Paris et des Departements, 77: 149-152

Ziegler, R.; Goebel, L.; Seidel, R.; Cucchiarini, M.; Pape, D.; Madry, H., 2016: Effect of open wedge high tibial osteotomy on the lateral tibiofemoral compartment in sheep. Part III: analysis of the microstructure of the subchondral bone and correlations with the articular cartilage and meniscus. First, to evaluate whether medial open wedge high tibial osteotomy (HTO) induces alterations of the microstructure of the lateral tibial subchondral bone plate of sheep. Second, to test the hypothesis that specific correlations exist between topog...

Pandian, N.G.; Kreis, A.; Brockway, B.; Isner, J.M.; Sacharoff, A.; Boleza, E.; Caro, R.; Muller, D., 1988: Ultrasound angioscopy: real-time, two-dimensional, intraluminal ultrasound imaging of blood vessels. American Journal of Cardiology 62(7): 493-494

Brahim, K.; Ray, D.T.; Dierig, D.A., 1998: Growth and yield characteristics of Lesquerella fendleri as a function of plant density. In field trials in 1992/93 and 1993/94 in central Arizona, USA, L. fendleri was grown at densities of 250 000, 500 000, 750 000 or 1 000 000 plants/ha, with unthinned controls of 1 658 000 plants/ha in 1992/93 and 1 500 000 plants/ha in 1993/94. I...

Girsberger, D., 1999: Education of the patient. Schweizer Monatsschrift für Zahnmedizin 109(8): 876-878