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Evaluation of alpha-cyano ethers as fluorescent substrates for assay of cytochrome P450 enzyme activity

, : Evaluation of alpha-cyano ethers as fluorescent substrates for assay of cytochrome P450 enzyme activity. Analytical Biochemistry 344(2): 183-192

We have previously reported the synthesis of four alpha-cyano-containing ethers based on 2-naphthaldehyde (2-NA) as cytochrome F 450 (P450) fluorescent substrates. Activity detection was based on the formation of fluorescent 2-NA following substrate hydrolysis. A major limitation of these substrates was the need to remove NADPH, a required cofactor for P450 oxidation, before measuring 2-NA fluorescence.


PMID: 16083846

DOI: 10.1016/j.ab.2005.06.032

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