Evaluation of alpha-cyano ethers as fluorescent substrates for assay of cytochrome P450 enzyme activity
Kang, K-Don.; Jones, P.D.; Huang, H.; Zhang, R.; Mostovich, L.A.; Wheelock, C.E.; Watanabe, T.; Gulyaeva, L.F.; Hammock, B.D., 2005: Evaluation of alpha-cyano ethers as fluorescent substrates for assay of cytochrome P450 enzyme activity. Analytical Biochemistry 344(2): 183-192
We have previously reported the synthesis of four alpha-cyano-containing ethers based on 2-naphthaldehyde (2-NA) as cytochrome F 450 (P450) fluorescent substrates. Activity detection was based on the formation of fluorescent 2-NA following substrate hydrolysis. A major limitation of these substrates was the need to remove NADPH, a required cofactor for P450 oxidation, before measuring 2-NA fluorescence.