geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Evaluation of alpha-cyano ethers as fluorescent substrates for assay of cytochrome P450 enzyme activity


, : Evaluation of alpha-cyano ethers as fluorescent substrates for assay of cytochrome P450 enzyme activity. Analytical Biochemistry 344(2): 183-192

We have previously reported the synthesis of four alpha-cyano-containing ethers based on 2-naphthaldehyde (2-NA) as cytochrome F 450 (P450) fluorescent substrates. Activity detection was based on the formation of fluorescent 2-NA following substrate hydrolysis. A major limitation of these substrates was the need to remove NADPH, a required cofactor for P450 oxidation, before measuring 2-NA fluorescence.

US$19.90

PMID: 16083846

DOI: 10.1016/j.ab.2005.06.032


Other references

Iwasaki, M.; Kumashiro, R.; Wada, T.; Maeyama, T.; Nagata, E.; Kawaguchi, M.; Abe, H.; Tanikawa, K., 1982: Effect of polymyxin B on endotoxemia in severe liver diseases. Nihon Shokakibyo Gakkai Zasshi 79(2): 241-248

Jones, A.; Winge, P.B.nes, A.; Cole, R.R.ssiter, J., 2002: Characterization and evolution of a myrosinase from the cabbage aphid Brevicoryne brassicae. The aphid myrosinase gene has been elucidated using Rapid Amplification of cDNA Ends--PCR. Sequencing has shown that aphid myrosinase has significant sequence similarity (35%) to plant myrosinases and other members of glycosyl hydrolase family 1 (...

Dlckstein, B.; Wolman, I.J.; Tan, C.; Slaughter, B.; Butson, H.; Cohen, B., 1952: Intravenous iron therapy in iron-deficiency anemia ot infancy and childhood. Eighty children, mostly aged between 6 and 30 months, received a total of 198 intravenous injections of saccharated iron oxide (feojectin) in doses of 25 to 100 mg. Anaemia was attributed in 66 to dietary Fe deficiency, in 14 to toxic and infectiv...

Farrand William R.; Frederickson G.C.I.I.I.; Stearns Carola H., 1995: Glacial lakes Maumee and Whittlesey; confirmation of a zone of zero glacial tilt. Abstracts with Programs - Geological Society of America 27(6): 169

Specht, A., 1981: The determination of tuber damage in testing potato varieties and potato harvesting machines. The depth of injured tissue was determined, at least four weeks after mechanical harvest, in tubers of 218 varieties which were peeled in a peeling machine. Fifty-three varieties had low susceptibility.

Hull, S., 2016: Patient-generated health data foundation for personalized collaborative care. Computers, Informatics, Nursing 33(5): 177-180

Forstner M.J., 1968: Serum analysis in pigs and cattle of varying ages. Zentralblatt fuer Veterinaermedizin Reihe A 15(1): 76-80

Heymann, G.A.; Lassahn, A.; Schönemann, C.; Salama, A., 2006: A novel HLA-A*680104 variant. Tissue Antigens 67(2): 169-170

Stavljenić, L.V.; Rajić, Z.; Cvorisćec, D.; Granić, P.; Mardesić, D.; Zaninović, M.; Devernay, Z., 1987: Changes in the composition of salivary amino acids, proteins and enzymes in children with phenylketonuria. Since children with phenylketonuria have a low incidence of caries, the aim of this study was to examine the changes occurring in the aminoacid and protein composition as well as in the composition of salivary enzymes, which might serve as biochem...

Sands, M.F., 2012: Localization of matrix metalloproteinase (MMP)-9 in lung tissue of a murine model of allergic asthma. MMP-9 (gelatinase B) is recognized in chronic obstructive pulmonary disease (COPD) and now asthma as playing a central role in matrix degradation in injury, as well as contributing to the remodeling process. The increasing focus on MMP-9 in human...