+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Expression of the Newcastle disease virus fusion protein in transgenic maize and immunological studies

, : Expression of the Newcastle disease virus fusion protein in transgenic maize and immunological studies. Transgenic Research 15(4): 455-463

Transgenic plants have been employed successfully as a low-cost system for the production of therapeutically valuable proteins, including antibodies, antigens and hormones. Here, we report the expression of the fusion (F) gene of the Newcastle disease virus (NDV) in transgenic maize plants. The expression of the transgene, driven by the maize ubiquitin promoter, caused accumulation of the F protein in maize kernels. The presence of the transgene was verified by Southern and western blots. Feeding chickens with kernels containing the F protein induced the production of antibodies, which conferred protection against a viral challenge. This protection was comparable to that conferred by a commercial vaccine. Possible uses of this plant-based F protein as a potential mucosal vaccine are discussed.


PMID: 16906446

DOI: 10.1007/s11248-006-0017-0

Other references

Arezki, H.; Boutchich, M.; Alamarguy, D.; Madouri, A.; Alvarez, Jé.; Cabarrocas, P.Roca.I.; Kleider, J-Paul.; Yao, F.; Hee Lee, Y., 2016: Electronic properties of embedded graphene: doped amorphous silicon/CVD graphene heterostructures. Large-area graphene film is of great interest for a wide spectrum of electronic applications, such as field effect devices, displays, and solar cells, among many others. Here, we fabricated heterostructures composed of graphene (Gr) grown by chemi...

Erspamer, V.; Testini, A., 1959: Observations on the release and turnover rate of 5-hydroxytryptamine in the gastrointestinal tract. The results from experiments on man, dogs and rabbits support the theory of the intestinal origin of blood 5-hydroxytryptamine (5-HT). It is suggested that gastric mucosa is probably the only source of extracerebral 5-HT and, consequently, of all...

Tumadzhanov I.I., 1971: Botanico geographical characteristics of high mountain dagestan as related to paleo geography of the pleistocene and holocene. Botanicheskii Zhurnal (St Petersburg) 56(9): 1239-1251

Xie, G.; Wang, X.; Liu, P.; Wei, R.; Chen, W.; Rajani, C.; Hernandez, B.Y.; Alegado, R.; Dong, B.; Li, D.; Jia, W., 2016: Distinctly altered gut microbiota in the progression of liver disease. Recent studies underscore important roles of intestinal microbiota and the bacterial lipopolysaccharides (LPS) production in the pathogenesis of liver disease. However, how gut microbiota alters in response to the development of steatosis and subs...

Rischbieth, J.R.; Sinclair, R.M.; Bussell, K.R.; Gunning, R.H., 1950: The paint-holding properties of Austrlaian timbers. An interim report on the results of two years' tests with the following Australian timbers: Araucaria cunninghamii, Eucalyptus marginata, E. pilularis, E. regnans, E. maculata group, Callitris glauca, Pinus radiata. A range of painting system...

Ellis, R.B.; Rapson, N.T.; Patrick, A.D.; Greaves, M.F., 1978: Expression of hexosaminidase isoenzymes in childhood leukemia. Because differences in hexosaminidase isoenzyme profiles of granulocytes and lymphoyctes suggested that such profiles might help to distinguish between various types of leukemia, was examined leukocyte extracts from 55 untreated children and 12 co...

Sanchez, J.; Svennerholm, A.M.; Holmgren, J., 1988: Genetic fusion of a non-toxic heat-stable enterotoxin-related decapeptide antigen to cholera toxin B-subunit. A decapeptide highly homologous to the STa Escherichia coli heat-stable enterotoxin and to several other heat-stable enterotoxins was fused genetically to the amino-end of the B-subunit of cholera toxin (CTB) and the hybrid protein gene expressed...

Fischer, M., 1970: Revision der nearktischen Aspilota-Arten der Sektion D and Erganzungen zu anderen Artengruppen. Annln naturh. Mus. Wien, 74: 91-127

Bhanji, N.H.; Margolese, H.C., 2004: Tardive dyskinesia associated with olanzapine in a neuroleptic-naive patient with schizophrenia. Canadian Journal of Psychiatry. Revue Canadienne de Psychiatrie 49(5): 343-343

Hamminga, G.M.; Mul, G.; Moulijn, J.A., 2007: Applicability of fiber-optic-based Raman probes for on-line reaction monitoring of high-pressure catalytic hydrogenation reactions. This study evaluates the applicability of fiber-optic-based Raman probes for on-line reaction monitoring of high-pressure catalytic hydrogenation reactions in batch autoclaves. First, based on trends in the strong intensity of the 945 cm(-1) C-O-C...