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Gene expression profiles of periodontal ligament cells treated with enamel matrix proteins in vitro: analysis using cDNA arrays


, : Gene expression profiles of periodontal ligament cells treated with enamel matrix proteins in vitro: analysis using cDNA arrays. Journal of Periodontology 75(11): 1539-1546

Background: A number of procedures have been used to achieve periodontal regeneration. Recently, enamel matrix derivative (EMD) has been the subject of significant basic and clinical investigations. The precise molecular events involved in EMD modulation of periodontal wound healing are not completely understood; however, cDNA microarray technology may enable rapid and accurate examination of EMD-mediated changes in gene expression in periodontal ligament (PDL) cells in vitro. The present study was undertaken to explore the selective effects of EMD on the activities of 268 cytokine, growth factor, and receptor genes in PDL. Methods: PDL cells were cultured in the absence and presence of EMD at a concentration of 100 mug/ml for 4 days. RNA was extracted and used to generate labeled cDNA probes. These were hybridized to cDNA arrays comprising 268 genes and exposed to x-ray films. Autoradiographs were digitized and analyzed. Results: Forty-six percent (125 of 268) of the tested genes were found to be expressed by the PDL cells. Of these 125 genes, 38 were differentially expressed by PDL cells which had been cultured in the presence of EMD. Among the 38, 12 were found to be downregulated, notably mostly inflammatory genes, whereas 26 genes demonstrated upregulation, many of these coding for growth factors and growth factor receptors. Conclusions: The present study has shown that EMD down-regulates the expression of genes involved in the early inflammatory phases of wound healing while simultaneously upregulating genes encoding growth and repair-promoting molecules. This may partly explain the apparent efficacy of EMD application in periodontal regeneration.

US$19.90

PMID: 15633332

DOI: 10.1902/jop.2004.75.11.1539


Other references

Searle, S.R., 1962: Bull sampling programmes in the artificial breeding of dairy cattle. An artificial breeding organization can maintain a steady supply of proven bulls for itself through the use of a bull-sampling programme. This involves choosing young, untried bulls on the basis of pedigree records and using them in the artificial...

Stanke Labesque, F.; Devillier, P.; Cracowski, J.L.; Caron, F.; Hardy, G.; Bessard, G., 2000: Nitric oxide enhances cysteinyl leukotrienes production in response to the calcium ionophore A23187 in aorta from spontaneously hypertensive rats but not from Wistar Kyoto rats. Fundamental & Clinical Pharmacology 14(3): 291, May-June

Vijayakumar, E.K.; Weidemann, M.J., 1976: Location of an oligomycin-insensitive and magnesium ion-stimulated adenosine triphosphatase in rat spleen mitochondria. 1. When rat spleen mitochondria are incubated with oxidizable substrates, added MgCl2 (greater than 150 muM free concentration) markedly stimulates state-4 respiration and lowers both the respiratory control and ADP/O ratios; this effect is revers...

Pickford, M., 1976: A new species of Taucanamo Mammalia Artiodadyla Tayassuidae from the Siwaliks of the Potwar Plateau, Pakistan. Pakistan Journal of Zoology, 81: 13-20

Wang, W.; Yu, J-Tai.; Zhang, W.; Cui, W-Zhen.; Wu, Z-Chen.; Zhang, Q.; Tan, L., 2013: Genetic association of SLC2A14 polymorphism with Alzheimer's disease in a Han Chinese population. Glucose uptake and metabolism are impaired in Alzheimer s disease (AD) brain, which appear to be a cause, rather than a consequence of neurodegeneration. Recently, the gene of the 14th isoform of subfamily A of solute carrier family 2 (<i>SL...

Foerster, C.O.; Jr., 1967: Sidegrafting of succulent grapevine tips onto established vines. A technique is described whereby plant breeders may bring vine seedlings into fruit production very rapidly. Seeds were sown in February, and after 70 days the tips of the seedlings were side-grafted onto established vines with a diameter of J in....

Hung, S.; Mcphee, S.; Jenkins, C.; Nguyen, K.; Fordham, D.; Ha, N., 1995: Dietary intake patterns of Vietnamese in California. To characterize dietary intake patterns among Vietnamese immigrants, we developed a Vietnamese-language nutrition survey using items from the Centers for Disease Control's Behavioral Risk Factor Surveillance survey and other sources. We surve...

Behura, BK.; Kar, SK., 1984: The Bhitarkanika Wild Life Sanctuary, Orissa. Pranikee, 5: 31-34

MacConachie, E., 1972: Serum hepatitis with polyarteritis nodosa--a personal account. Nursing Times 68(34): 1070-1072

Jadwiszczak A., 1987: Contribution to the knowledge of the epilachninae coleoptera coccinellidae of the australian region with a description of a new species. Nineteen species of the Epilachnine of Australian Region have been studied, including Epilachna slipinskii n. sp. Male genitalia of Epilachna haematomelas (Boisd.) and Afidentula aruensis (Crotch) have been described for the first time.