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Genetic transformation of barley to modify expression of a 13-lipoxygenase


, : Genetic transformation of barley to modify expression of a 13-lipoxygenase. Acta Biologica Szegediensis 49(1-2): 33-34,31

Immature scutella of barley were transformed with cDNA coding for a 13-lipoxygenase of barley (LOX-100) via particle bombardment. Regenerated plants were tested by PAT-assay, Western-analysis and PCR-screening. Immunocytochemical assay of TO plants showed expression of the LOX cDNA both in the chloroplasts and in the cytosol, depending on the presence of the chloroplast signal peptide sequences in the cDNA.

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