geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Hypoxia enables B19 erythrovirus to yield abundant infectious progeny in a pluripotent erythroid cell line


, : Hypoxia enables B19 erythrovirus to yield abundant infectious progeny in a pluripotent erythroid cell line. Journal of Virological Methods 121(2): 145-153

B19 may cause mild to severe clinical manifestations. Owing to the remarkable tropism of B19 for red blood cell progenitors, there is a lack of satisfactory cell lines fully permissive for B19. Because the local oxygen pressure may influence viral replication, we used hypoxia to improve the sensitivity of our infectivity assay in order to link B19 DNA detected by PCR to the presence of infectious B19 particles in plasma. Plasma samples and the WHO International Standard for B19 DNA detection by PCR were used to infect the pluripotent human erythroid cell line KU812F under different oxygen pressures. Specific human anti-B19 IgG was found to reduce infectivity. Low oxygen pressure led to higher yields of infectious B19 progeny and to a higher level of viral transcription than observed under normoxia. This sensitive infectivity assay is a promising model for studying B19 biology, identifying neutralising antibodies, and evaluating new virus inactivation methods. Copyright 2004 Elsevier B.V. All rights reserved.

US$19.90

PMID: 15381351

DOI: 10.1016/j.jviromet.2004.06.010


Other references

Chu Dong; Zhang YouJun; Cong Bin; X.B.oYun; W.Q.ngJun; Zhu GuoRen, 2005: Sequence analysis of mtDNA COI gene and molecular phylogeny of different geographical populations of Bemisia tabaci (Gennadius). Bemisia tabaci(Gennadius) is a serious pest in many cropping systems worldwide and occurs in different biotypes. The mtDNA COI gene of the 12 Bemisia tabaci (Gennadius) populations from different regions and countries were analyzed. Based on mtDNA...

Schmidt R.A.; Jokela E.J.; Allen J.E.; Belanger R.P.; Miller T., 1990: Association between fusiform rust incidence and criff soil classification for slash pine plantations in the coastal plain of florida and georgia usa. The association between fusiform rust incidence in young slash pine plantations and the CRIFF (Cooperative Research in Forest Fertilization) soil classification system in the Coastal Plain of Florida and Georgia was examined in two independent dat...

Coatrieux, J-Louis., 2003: Signal processing and physiological modeling--part 1: Surface analysis. Signal processing offers a wide spectrum of theories, methods, and algorithms for addressing a variety of problems ranging from noise reduction, restoration, detection (of events or changes), spatiotemporal dynamics estimation, source localization...

Cohen, M.; Kubak, B., 1997: Mycoplasma hominis meningitis complicating head trauma: case report and review. Clinical Infectious Diseases 24(2): 272-273

Andueza, D.; Jestin, M.; Meignant, V.; Michalet Doreau, B., 2004: Using of NIRS to estimate the leaf:stem ratio in different botanical species of seminatural grasslands. Leaf:stem ratio of forages is an important factor affecting quality and forage intake by ruminants. However, its manual determination is a tedious, time consuming and costly process. The objective of this study was to evaluate the suitability of n...

Desmond M.; Krainz P.A., 1991: Sample control for an expanding laboratory testing service. Transfusion (Bethesda) 31(8 SUPPL): 77S

Tankoff, W.E., 1925: About semiotics and therapie of Ascaridosis. Russian Journal of Tropical Medicine, 1924: 48-55

Yonetani Hiroshi; Miyashita Michio, 1968: Exploration for helium resources in the vicinity of Isobe-machi, Gumma prefecture. Natural gas sampling-analyses, relatively low helium concentrations, Japan.

Frantz, A.G.; Kleinberg, D.L.; Noel, G.L.; Suh, H.K., 1973: Effects of neuroleptics on the secretion of prolactin and growth hormone. Prolactin was detected by bioassay in all of 9 patients on chronic tranquillizing drug therapy (12 or 24 mg perphenazine, 12 mg perphenazine + 100 mg amitriptyline, 6 mg fluphenazine, 1.5 or 2 g chlorpromazine, 150 mg imipramine or 4.5 mg haloperi...

Robson, J.S., 1947: Plasma and corpuscular cholesterol in sprue. Blood from 5 proved cases of sprue on a diet containing 90 g. of fat per day was analysed. Plasma cholesterol showed values between 103 and 193 mg. per cent. with an average of 146. Corpuscular cholesterol was not altered from normal. Plasma fatty...