geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification and characterization of phosphorylated proteins in the human pituitary


, : Identification and characterization of phosphorylated proteins in the human pituitary. Proteomics 4(3): 587-598

Post-translational modifications of proteins from the human pituitary gland play an important role in the regulation of different body functions. We report on the application of a liquid chromatography-tandem mass spectrometry (MS/MS) based approach to detect and characterize phosphorylated proteins in a whole human pituitary digest. By combining an immobilized metal affinity column-based enrichment method with MS/MS conditions that favor the neutral loss of phosphoric acid from a phosphorylated precursor ion, we identified several previously undescribed phosphorylated peptides. The identified peptides were matched to the sequences of six pituitary proteins: the human growth hormone, chromogranin A, secretogranin I, 60S ribosomal protein P1 and/or P2, DnaJ homolog subfamily C member 5, and galanin. The phosphorylation sites of these important regulatory proteins were determined by MS/MS and MS(3) analysis.

US$19.90

PMID: 14997482

DOI: 10.1002/pmic.200300584


Other references

Foster, R.S., 2000: Trends in Medicare Expenditures and Financial Status, 1966-2000. In this article, the author reviews expenditure growth trends over Medicare's 35-year history and comments on how the program's long-range financial outlook has changed over time. The author focuses on the various legislative, economic,...

Francsics, P.; Parti, M.; Topar, J.; Dugmanics, I., 1991: Optimum utilization of cooling air in cereal drying. A simulation model was used to study the effect of operational parameters on the output of grain drying equipment. In single-or double-flow equipment moisture removal was considerably reduced when the cooling air flow and initial temp. were increa...

Kaminska J.; Gora J., 1988: Syntheses and odor characteristic of beta terpinyl esters. Perfumer & Flavorist 12(6): 53-54, 56, 1987

Wang, L.; Wu, Y-Song.; Tang, Y-Ping.; Li, M., 2005: Detection of SARS-associated coronavirus N protein by time-resolved fluoroimmunoassay. To develop a method for quantitative detection of severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) N protein by timed-resolved fluoroimmunoassay (TRFIA). Using a monoclonal antibody (mAb) against SARS-CoV N protein, scree...

Willis, C.K.R.; Brigham, R.Mark.; Geiser, F., 2006: Deep, prolonged torpor by pregnant, free-ranging bats. Many mammals save energy during food shortage or harsh weather using controlled reductions in body temperature and metabolism called torpor. However, torpor slows offspring growth, and reproductive individuals are thought to avoid using it because...

Knapp, S.E., 1966: Distribution of Angiostrongylus cantonensis larvae in the giant African snail, Achatina fulica. Only 1.3% and 8% of the infective larvae of Angiostrongylus cantonensis from 5 and 8 Achatina fulica respectively were recovered from the foot.

Greenberg, D.; Monaghan, J.J.M.; Dietz, M.; Marquardt, T.; McAlpine, D., 2017: Influence of envelope waveform on ITD sensitivity of neurons in the auditory midbrain. Interaural time differences (ITDs) conveyed by the modulated envelopes of high-frequency sounds can serve as a cue for localizing a sound source. Klein-Hennig et al. (<i>J Acoust Soc Am</i> 129: 3856, 2011) demonstrated the envelope at...

Greenberger, J.S., 1984: Recent modifications of technique in human long-term bone marrow cultures. Kroc Foundation Series 18: 119-131

Adlington, R., M.; Baldwin, J., E.; Challis, G., L., 1998: Rate enhancement in the reduction of (2,3)-alpha- and (2,3)-beta-methylenepenam beta-sulfoxides. The ease of reduction of (2,3)-alpha-methylenepenam beta-sulfoxides, (2,3)-beta-methylenephenam beta-sulfoxides and bicyclic penam beta-sulfoxides to the corresponding sulfides, using potassium iodide and trifluoroacetic anhydride in acetone or N,...

Bleiler, R.E.; Yearick, E.S.; Springer, N.S.; Stevens, H., 1960: Serum lipids as related to previous diet. FED PROC 19(1 Pt 1): 237