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Improved preservation of human red blood cells by lyophilization

, : Improved preservation of human red blood cells by lyophilization. Cryobiology 51(2): 152-164

The lyophilization of human red blood cells has important implications for blood transfusion in clinical medicine. In this study, sugars, human serum albumin, polyvinylpyrrolidone, and dimethyl sulfoxide were used as protective reagents for the lyophilization of red blood cells. Freezing temperature, shelf temperature, and the rehydration conditions were optimized. The results showed that extracellular disaccharides, especially trehalose, did not increase the recovery of hemoglobin. However, when the concentration of human serum albumin was higher than 25%, it had a considerable protective effect on the recovery of lyophilized red blood cells; the cellular hemoglobin recovery was over 70%, which was significantly higher than that in the group without human serum albumin (P < 0.01). As the concentration of polyvinylpyrrolidone was increased, the extent of vitrification also increased. But when the concentration of polyvinylpyrrolidone was over 40%, the resulting concentration of free hemoglobin was over 1 g/L, which was significantly higher than that with 40% (P < 0.01). When lyophilization was carried out after freezing at different temperatures, the recovery of cells and hemoglobin was 70-80% and there were no significant differences among the five groups. When the shelf temperature was higher than -30 degrees C, the samples were partly collapsed, but when the shelf temperature was lower than -30 degrees C the recovery of cells in the -40 and -45 degrees C groups was significantly higher than in the -30 and -35 degrees C groups (P < 0.05). The recovery of cells and hemoglobin after lyophilization and rehydration in solutions containing low concentrations of polymers was over 80%, which is significantly higher than the other groups (P < 0.01). In addition, when the temperature was higher than 25 degrees C, the concentration of free hemoglobin was significantly lower than it was at 4 degrees C (P < 0.01). In conclusion, our study showed the lyophilization of red blood cells is feasible. Disaccharides have no protective effect on lyophilized cells when they are only extracellular and extensive vitrification may be not beneficial. Although the recovery of cells after lyophilization and rehydration by our method was over 70%, the ultrastructure of the cells may be compromised and some hemolysis does still exist. Further research is required. (c) 2005 Elsevier Inc. All rights reserved.


PMID: 16095589

DOI: 10.1016/j.cryobiol.2005.06.002

Other references

Tielen, Mjm, 1977: Development of automation requirements of swine husbandry. Bedrijfsontwikkeling 8 (9) 783-785

Wang, J.; Guo, Y.; Liang, Z.; Hao, J., 2003: Amino acid composition and histopathology of goat teeth in an industrial fluoride polluted area. Amino acid analyses and histopathological observations were made on decalcified teeth of goats living in an industrial fluoride polluted area. The content of glycine, glutamic acid, phenylanine, and proline of the fluorosed teeth was significantly...

Sato, S., 1950: Contributions to the study on cultivation of leprosy bacilli; a re-examination of Prof. Nakamura's method and on the behavior of bacilli implanted in various nutrient liquid media with some accessory substances. Science Reports of the Research Institutes, Tohoku University. Ser. C, Medicine. Tohoku Daigaku 2(2): 1-7

Postgate, J.R., 1982: The fundamentals of nitrogen fixation. The following are discussed: (a) dinitrogen fixation and the nitrogen cycle, (b) nitrogen-fixing bacteria, (c) enzymology, (d) physiology, (e) genetics, (f) ecology, (g) origin and evolution, (h) chemistry and mechanism, and (i) nitrogenase action...

Cox, N.; Blankenship, L., 1975: Comparison of rinse sampling methods for detection of salmonellae on eviscerated broiler carcasses. Journal of food science: 40 (6) 1333-1334

Shimizu, M.; Tanabe, T.; Fukae, M., 1979: Proteolytic enzyme in porcine immature enamel. A proteolytic enzyme cleaving the main component of enamel proteins obtained from immature enamel has been purified from a soluble extract of porcine immature enamel. It is optimally active around pH 6 against enamel protein. It is completely inhi...

Fukushima, K.; Nishimi, Y.; Mori, K.; Takeda, J., 1995: Central effect of epidurally administered dexmedetomidine in man. Anesthesiology (Hagerstown) 83(3A): A82

Martenet, B.; Mueller, C., 1986: Does one become an alcoholic after age 60?. The authors have examined all the patients which were older than 60 years and had been hospitalised for alcoholism over a span of 10 years and within a certain region. The study of their files shows that more than 70% had a previous history of alc...

Carter, W.C.; Scott, R.L.; Magat, M., 1946: The viscosity-molecular weight relation for natural rubber. The intrinsic viscosity-osmotic molecular weight relation for Hevea was detd., corresponding to the equation [[image]] = 5.02 X 10-4 [image] 0.667. Hevea from ribbed smoked sheet was fractionated by fractional pptn. by methanol from toluene solns....

Edwards, A.M.; Braun, P.E., 1988: Gene expression of the central and peripheral nervous system myelin membrane 2', 3'-cyclic nucleotide 3'-phosphodiesterase in development. Determination of gene expression for the 2', 3'-cyclic nucleotide 3'-phosphodiesterase (CNP) during development shows that the amount of translatable RNA encoding this enzyme increases in parallel with myelination in the mouse brain...