geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

In situ hybridization: Basic approaches and recent development


, : In situ hybridization: Basic approaches and recent development. Journal of Histotechnology 27(1): 53-67, March

In situ hybridization (ISH) allows for the histological and cytological localization of DNA and RNA targets. ISH has been useful as a research tool, and recent studies have used this technique in the diagnostic pathology laboratory and in microbiology for the tissue localization of infectious agents. However, the application of ISH techniques can be limited by their inability to detect targets with low copies of DNA and RNA. During the last few years, several strategies have been developed to improve the sensitivity of ISH by amplification of either target nucleic acid sequences before ISH or signal detection after the hybridization is completed. Current approaches involving target amplification as in situ PCR, and signal amplification as tyramide deposition are reviewed with emphasis on their applications to bright field microscopy. Application of some of these techniques has extended the utility of ISH in diagnostic pathology and in research, because of the ability to detect targets with low copy numbers of DNA and/or RNA.

US$19.90

DOI: 10.1179/his.2004.27.1.53


Other references

Allan, G.; Williams, A.; Rabinowicz, P.D.; Chan, A.P.; Ravel, J.; Keim, P., 2008: Worldwide genotyping of castor bean germplasm (Ricinus communis L.) using AFLPs and SSRs. Worldwide genetic diversity in 200 individuals comprising 41 castor bean accessions was assessed using amplified fragment polymorphisms (AFLPs) and simple sequence repeats (SSRs). We found that, despite surveying five continents and 35 countries,...

Rivas, J.A.; Owens, R.Y.; Calle, P., 2001: Eunectes murinus (green anaconda). Juvenile predation. Herpetological Review 32(2): 107-108

Türkçüoğlu, P.; Aydemir, O.; Oztürkmen, C.; Yldrm, H., 2008: Pontine gaze deviation and face turn relieved by eye muscle surgery. A 34-year-old woman developed a bilateral horizontal gaze palsy, left gaze deviation, and right face turn consequent to a pontine hemorrhage. A bilateral horizontal recession and resection of extraocular muscles in both eyes (Parks procedure) elim...

Borland C.; Cox Y.; Higenbottam T.W., 1991: Reduction in pulmonary capillary blood volume qc measured by simultaneous nitric oxide and carbon monoxide diffusing capacity dlno dlco in patients with primary hypertension. American Review of Respiratory Disease 143(4 PART 2): A760

Backman, E.Louis, 1912: Der osmotische Druck bei einigen Wasserkafern. ArchPhysiol. Bonn: 93-114

Constantineanu, I.; Constantineanu, R.; Lungu Constantineanu, C.S.efan, 2001: New ichneumonid species (Hymenoptera, Ichneumonidae) for the Romanian fauna. Entomologica Romanica2001): 89-90

Zelin, S.W.lton, J., 1986: An instrument to detect estrus in dairy cows. American Society of Agricultural Engineers Microfiche collection: (fiche no 86-3505)

Winter, R.N.; Kramer, A.; Borkowski, A.; Kyprianou, N., 2001: Loss of caspase-1 and caspase-3 protein expression in human prostate cancer. Activation of the caspase cascade is involved in the execution of apoptosis in a variety of cellular systems. Recent studies demonstrated that caspase-1 activation was required for human prostate cancer cells to undergo apoptosis in response to tr...

Yang, G.; Wan, X.; Liu, Y.; Li, R.; Su, Y.; Zeng, X.; Tang, J., 2016: Luminescent Poly(vinyl alcohol)/Carbon Quantum Dots Composites with Tunable Water-Induced Shape Memory Behavior in Different pH and Temperature Environments. Luminescent water-induced shape memory polymer (SMP) composites with tunable shape recovery rate are developed by blending poly(vinyl alcohol) (PVA) and carbon quantum dots (CQDs). The oxygen and active hydrogen-rich CQDs can serve as extra physic...

Muller, Yves., 1999: Ornithological bibliography of Lorraine Bibliographie dornithologie Lorraine. Ciconia (Eguelshardt): 1-578 Numero special