geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

In vitro susceptibility of macrophages to porcine reproductive and respiratory syndrome virus varies between genetically diverse lines of pigs


, : In vitro susceptibility of macrophages to porcine reproductive and respiratory syndrome virus varies between genetically diverse lines of pigs. Viral Immunology 18(3): 506-512

Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be responsible for financial losses in the swine industry worldwide. It remains undetermined whether genetic variability of the host in susceptibility to PRRSV exists and if this variability can be exploited to help control this important disease. The objective of this study was to determine if an in vitro flow cytometry (FACS) assay that detects the percentage of monocyte-derived macrophages (MDM) infected with PRRSV could be utilized to demonstrate genetic variability in the susceptibility between distinct lines of pigs. Over 400 growing pigs from six genetic lines maintained in a single commercial breeding herd were screened using an in vitro FACS assay. From this initial screening, two genetically diverse lines of pigs that were also divergent in their FACS results were selected for further study. An additional 264 pigs from these two lines were subsequently tested for in vitro susceptibility to PRRSV. As in the preliminary screening, the Large White line had significantly higher average percent positive MDM over the Duroc-Pietrain synthetic line. This report suggests a genetic component for susceptibility to PRRSV exists and that the in vitro assay may be useful in predicting the relative susceptibility to PRRSV in large groups of animals.

US$19.90

PMID: 16212529

DOI: 10.1089/vim.2005.18.506


Other references

Holcik, Jurai., 1996: Geographic distribution of lampreys (Petromyzontiformes) in the Middle and Lower Danube (between Austria and the Black Sea). Fischoekologie. 1995( ); 8: 23-30

Finney J.R.; Harding J.B., 1982: The susceptibility of simulium verecundum diptera simuliidae to isolates of bacillus thuringiensis serotype 10 darmstadiensis. Mosquito News 42(3): 434-435

Chambon-Savanovitch, C.; Felgines, C.; Farges, M.C.; Raul, F.; Cézard, J.P.; Davot, P.; Vasson, M.P.; Cynober, L.A., 1999: Comparative study of glycine, alanine or casein as inert nitrogen sources in endotoxemic rats. Pharmacological effects of dietary amino acids (AA) and peptides must be compared to an isonitrogenous control that is as inert as possible. To establish a rationale for the choice of such a control, potential metabolic and nutritional effects of...

Nitahara, J.A.; Valencia, M.; Bronstein, M.A., 2006: Medical case management after laminectomy or craniotomy: do all patients benefit from admission to the intensive care unit?. To define severity of illness to identify most effectively patients for whom admission to the intensive care unit (ICU) is unnecessary, the authors performed a retrospective cost-effectiveness analysis. The authors studied the records of 113 patie...

Gabriel, R., 1992: Molar or mass?. Central African Journal of Medicine 38(10): 420-421

Chaves S.; M.; Gonzalez G.; F., 1955: Manurial trials in clay-silt soils in Seville Province. Wheat cultivation on non-irrigated land. Results from manurial trials with wheat grown in soil poor in both nutritive elements and organic matter indicated that applications of P gave highly significant increases in yield, while N gave significant increases. K manuring did not increase y...

Sprenger, A.M., 1932: The relation between growth and productivity. This article is a dissertation in semi-popular form in support of the theory that a fruit tree can and should be in luxuriant growth without suffering any reduction of fruitfulness.

Lu, H.P.ter; Chen, Yu, 2001: Single-molecule spectroscopy study of the mechanisms and dynamics of non-covalent and covalent molecular interactions. Biophysical Journal 80(1 Part 2): 340a, January

Depamphilis, M.L.; Berg, P., 1975: Requirement of a cytoplasmic fraction for synthesis of sv 40 dna in isolated nuclei. Journal of Biological Chemistry 250(11): 4348-4354

Brahma K.C.; Dash B.D.; Kara T.C.; Patnaik B.K., 1983: Effect of castration and testosterone treatment on the morphometric growth organ weight and tissue biochemical parameters of young male garden lizard calotes versicolor. The morphometric parameters of growth (body weight and snout-vent length) of young lizards were not affected significantly either by castration or by testosterone treatment during a period of 2 wk. But alcohol, the vehicle through which the hormon...