geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Inhibition of BRCA1 in breast cell lines causes the centrosome duplication cycle to be disconnected from the cell cycle


, : Inhibition of BRCA1 in breast cell lines causes the centrosome duplication cycle to be disconnected from the cell cycle. Oncogene 25(2): 298-303

BRCA1-dependent ubiquitination activity regulates centrosome number in several tissue culture cell lines derived from breast cells. In these experiments, we asked how BRCA1 inhibits centrosome amplification. In general, supernumerary centrosomes can accumulate by three mechanisms: (1) failed cytokinesis and the accumulation of centrosomes by duplication in a repeated S-phase of the cell cycle, (2) disruption of the licensing of centrosome doubling such that they duplicate at inappropriate times in the cell cycle, or (3) fragmentation of the centrosomes. In this study, we found that inhibition of BRCA1 caused premature separation of centrioles and reduplication. By blocking cells in early S-phase before centrosome amplification secondary to BRCA1 inhibition could occur and then releasing, we found that inhibition of BRCA1 caused centrosome amplification between late S-phase and G2/M before the cell divided. These results suggest that normal BRCA1 function is critical in these cell lines to prevent centriole separation and centrosome reduplication before mitosis.

US$19.90

PMID: 16170356

DOI: 10.1038/sj.onc.1209028


Other references

Shen, J.; Jiang, Q-wu., 2015: The changing gaps of life expectancy on genders in urban cities of China, from 2005 to 2010. To analyze the gender difference of life expectancy in urban people of China and to explore both age-specific and cause-specific contributions to the changing differences in life expectancy on genders. Data on life expectancy (male and female) and...

Myakotina O.L.; Tsofina L.M., 1989: Effect of metavanadate on the membrane potential of mitochondria. Metavanadate, like all usual inhibitors of respiratory chain of mitochondria induces a drop in the membrane potential (.DELTA.psi.). However, in the presence of 5.cntdot.10-5-1.cntdot.10-3 M EDTA the potential decrease is preceded by its increase...

Datta, M.; Choudhuri, D.K.; Dasgupta, B., 1984: Response of blackflies (Diptera: Simuliidae) to certain coloured light sources. Attraction to light in 6 species of simuliids was tested in Darjeeling, India, using lights of 5 different colours. All 6 species appeared to be more attracted to snow-white light than to any other colour, the order of preference being white, chro...

Montan, S.; Anandakumar, C.; Joseph, R.; Arulkumaran, S.; Ng, S.C.; Ratnam, S.S., 1996: Fetal and neonatal haemodilution associated with multiple placental chorioangioma: case report. A pregnancy with polyhydramnios and abnormal antepartum fetal heart rate pattern was found to have multiple placental haemangiomas. Multiple placental haemangiomas can give rise to fetal cardiac failure due to a hyperdynamic circulation or fetal a...

Hohne, J., 1983: The influence of energy and environmental problems on the design of dairies. Possibilities for reducing energy consumption and improving the environment are discussed with specific reference to purification of smoke, purification of waste water and the needs for working out energy balances and total planning so that every...

Podol'skaya, L.A.; Solov'ev, N.A., 1989: Morphological changes of electrically inactive afferent myelinated nerve fibers of the living bushlike interoceptor. Neuroscience and Behavioral Physiology 19(4): 318-324

Batista, C.Augusto.Marques.; Colleoni Neto, R.; Lopes Filho, G.de.Jesus., 2008: Comparative study of the healing process of the aponeurosis of the anterior abdominal wall of rats after wound closure using 3-0 nylon suture and N-butil-2-cyanoacrylate tissue adhesive. To investigate the healing process of the aponeurosis of the anterior abdominal wall of rats, comparing two different materials for wound closure: 3-0 nylon suture and tissue adhesive N-butyl-2-cyanoacrylate. Forty-four Wistar rats were randomly d...

Chen, Y-Lien.; Yeh, S-Hsing.; Lin, T-Wei.; Chen, C-Chu.; Chen, C-Shuh.; Kuo, C-Feng., 2016: A 90-Day Subchronic Toxicity Study of Submerged Mycelial Culture of Cordyceps cicadae (Ascomycetes) in Rats. Cordyceps cicadae is a parasitic fungus that hibernates inside a host (Cicada flammata Dist.) and then grows its fruiting body on the surface of the insect. The complete insect/fungus combination of C. cicadae has been widely applied in Chinese tr...

Jaeckel, E., 1964: Contribution To The Clinical Picture Of Diaphragmatic Hernia. Zentralblatt für Chirurgie 89: 707-717

Fuereder, M.; Panke, S.; Bechtold, M., 2012: Simulated moving bed enantioseparation of amino acids employing memory effect-constrained chromatography columns. Teicoplanin aglycone-based chromatography columns (Chirobiotic TAG) enable amino acid enantioseparation with aqueous mobile phases, which perfectly accommodates the distinct hydrophilicity of most amino acids. Therefore, this stationary phase cons...