geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Insulin and IGF-I phosphorylate eNOS in HUVECs by a caveolin-1 dependent mechanism


, : Insulin and IGF-I phosphorylate eNOS in HUVECs by a caveolin-1 dependent mechanism. Biochemical and Biophysical Research Communications 337(3): 849-852

Caveolae are plasmamembrane regions which take part in the regulation of intracellular trafficking and signaling of tyrosine kinase receptors. Insulin and IGF-I receptors and their intracellular substrates localize in caveolae. Also eNOS is targeted to caveolae and caveolin-1, the major caveolar protein, acts as a regulator of eNOS activity. Since Insulin and IGF-I phosphorylate and activate eNOS, we investigated the role of caveolin-1 in Insulin and IGF-I stimulated eNOS activity. Here we show that: (1) in human endothelial cells, Insulin and IGF-I stimulate eNOS phosphorylation in a different manner both qualitatively and quantitatively; (2) caveolin-1 down regulation abolishes Insulin and IGF-I stimulated eNOS phosphorylation. These results suggest that caveolae could represent an intracellular site that contributes to differentiate IR and IGF-IR activity, and demonstrate the role of caveolin-1 in the eNOS activation by Insulin and IGF-I. (c) 2005 Elsevier Inc. All rights reserved.

US$19.90

PMID: 16225848

DOI: 10.1016/j.bbrc.2005.09.125


Other references

Cherkin, A., 1979: Farm or laboratory for male chicks?. Nature 281(5732): 522-522

Lippestad, C.; Sivertssen, E.; Skjaeggestad, O., 1977: The prognostic importance of age, sex and previous myocardial infarction. Journal of the Oslo City Hospitals 27(10): 129-130

Brown, N.M.; Benke, G.M., 1977: Safety testing of alkyl poly ethoxylate nonionic surfactants part 2 subchronic studies. The alkyl polyethoxylates CH3.cntdot.[CH2]11-12.cntdot.[OCH2.cntdot.CH2]6.cntdot.OH (or C13E6) and CH3.cntdot.[CH2]13-14.cntdot.[OCH2.cntdot.CH2]7.cntdot.OH(orC14E7) were evaluated for subchronic oral toxicity (using rats) and percutaneous toxicit...

Poli, E., 1978: Leaf area index and related problems. Informatore botanico italiano 10(1): 181-187

Strom, E.H.; Fogazzi, G.B.; Banfi, G.; Pozzi, C.; Mihatsch, M.J., 1994: Light chain deposition disease of the kidney: Morphological aspects in 24 patients. Renal biopsies and autopsy specimens of 23 patients with light chain deposition disease (LCDD) and one with only heavy chain deposits, were studied by light (LM) and electron microscopy (EM) as well as immunohistology (IH). Thirteen patients had m...

Bowen, D.T.; Wu, P.S.an; Talathi, J.; Smith, G.; Hay, A.; Keenan, N.; Thomas, G.E., 2003: Prior to presentation of pre-leukaemia Blood count abnormalities and the latency of disease development. Leukemia Research 27(Supplement 1): S4-S5, May

Kinoshita, T.; Fukutomi, T.; Kubochi, K., 2004: Magnetic resonance imaging of benign phyllodes tumors of the breast. Magnetic resonance imaging (MRI) has the potential to become a useful adjunct in breast imaging. Contrast-enhanced breast MRI has demonstrated a high sensitivity in the detection of benign and malignant breast disease. Our study aimed to correlate...

Tadgell, A.J., 1946: A hardy alien orchid. Epidendrum radicans var. O'brieni-anum, native of Brazil, has "gone bush" in Queensland. The orchid has a long flowering period; suitable for cultivation under greenhouse conditions.

Mcclanahan R.H.; Thomassen D.; Slattery J.T.; Nelson S.D., 1989: Metabolic activation of r dextro pulegone to a reactive enonal that covalently binds to mouse liver proteins. Pulegone, a naturally occurring hepatotoxin, is metabolically activated to chemically reactive intermediates that are capable of covalent binding to cellular protein. Studies in vivo and in vitro with inhibitors and inducers of cytochrome P-450 de...

Magnani, G.C., 1969: Microradiographic aspects of bone in the aged. Annali di Radiologia Diagnostica 42(6): 482-494