+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Lentiviral gp34/OX40L gene transfer into dendritic cells facilitates alloreactive CD4+ T-cell response in vitro

, : Lentiviral gp34/OX40L gene transfer into dendritic cells facilitates alloreactive CD4+ T-cell response in vitro. International Journal of Hematology 79(4): 377-383

Gene-modified dendritic cells (DCs) are promising targets for cancer immunotherapy. In this study, we demonstrated that lentiviral transduction of DCs with the gp34/OX40L gene, one of the costimulatory molecules, facilitates alloreactive CD4+ T-cell response in vitro. We achieved a 20% to 40% efficiency of gp34/OX40L gene transfer into DCs by the lentiviral vector, and lentiviral gp34/OX40L gene transfer did not alter the surface phenotype of either immature or mature DCs, suggesting that expression of gp34/OX40L did not induce the maturation of immature DCs and that gp34/OX40L-transduced DCs could fully differentiate into mature DCs. gp34/OX40L gene transfer facilitated an allogeneic CD4+ T-cell response in vitro by mature DCs but not by immature DCs. Dose escalation of the transgene induced an increasing amount of gp34/OX40L expression, leading to an increasing level of up-regulation of the allogeneic CD4+ T-cell response. The addition of anti-gp34 monoclonal antibody totally abrogated this up-regulation. These results suggest that this facilitation of allogeneic CD4+ T-cell response is specifically dependent on gp34/OX40L expressed on transduced DCs. Taken together, our findings show that gp34/OX40L plays an important role in allogeneic CD4+ T-cell activation by DCs and that lentiviral gp34/OX40L gene transfer into DCs may be a useful strategy for cancer immunotherapy. Copyright 2004 The Japanese Society of Hematology.


PMID: 15218969

DOI: 10.1532/ijh97.03120

Other references

Marriott, J., 1968: Regulation of porphyrin synthesis. Biochemical Society Symposium 28: 61-74

Bessesen, M.T.; Adams, J.C.; Radonovich, L.; Anderson, J., 2016: Disinfection of reusable elastomeric respirators by health care workers: A feasibility study and development of standard operating procedure. This was a feasibility study in a Department of Veterans Affairs Medical Center to develop a standard operating procedure (SOP) to be used by health care workers to disinfect reusable elastomeric respirators under pandemic conditions. Registered a...

Mothes, B.; D.C.mpos, M.A., 2004: Biemna trisigmata sp n, a new sponge from the north coast of Brazil Demospongiae, Poecilosclerida. A new species of Biemna Gray, 1867, B. trisigmata sp. n., is described from the northern Brazilian coast, Amapa State. The new species is compared to other tropical West Atlantic species of Biemna, from which it differs in having three categories...

Madeux, B.; Pons, B.; Elkoun, K.; Thiery, G., 2016: Uraemic frost: a clinical manifestation of severe uraemia. Postgraduate Medical Journal 92(1090): 491-491

Schujman, E.; Shomrat, N.; Loewenthal, M.; Bartal, A., 1976: Fibrosarcoma of the dura mater. Harefuah 91(10): 335-336

Quiroga, J.; Gálvez, J.; Abonia, R.; Insuasty, B.; Ortíz, A.; Cobo, J.; Nogueras, M., 2014: Highly efficient and diastereoselective synthesis of new pyrazolylpyrrolizine and pyrazolylpyrrolidine derivates by a three-component domino process. Diastereoselective reactions between 4-formylpyrazoles, N-substituted maleimides and glycine derivates led to new series of pyrazolyldipyrrolo [3,4-a:3',4'-f]pyrrolizines and pyrazolylpyrrolo[3,4-c]pyrroles in good yields. The reactions...

Hartman R.D.; Petersen S.L.; Barraclough C.A., 1988: Lhrh neurons have limited responsiveness to norepinephrine ne in estrogen treated ovariectomized rats. Society for Neuroscience Abstracts 14(1): 439

Newman, B.; Schmitz, M.; Gawande, R.; Vasanawala, S.; Barth, R., 2014: Perforated appendicitis: an underappreciated mimic of intussusception on ultrasound. We encountered multiple cases in which the US appearance of ruptured appendicitis mimicked intussusception, resulting in diagnostic and therapeutic delay and multiple additional imaging studies. To explore the clinical and imaging discriminatory f...

Fraser, D.; Rushen, J., 1993: A colostrum feeder for newborn lambs. Newborn lambs were studied in a series of experiments to identify simple cues that elicit teat-seeking behaviour, and to incorporate these cues into a device that would guide lambs to find and suck from a colostrum bottle. In six experiments, lamb...

Ikegawa, S., 2010: Genetic basis for skeletal disease. Genetic factors of osteoarthritis with particular reference to skeletal dysplasia. Osteoarthritis (OA) is a degenerative disease of articular cartilage. Etiologically, it is classified as primary (idiopathic) and secondary forms. Primary OA is a polygenic disease based on genetic and environmental factors. Identification of its...