geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Membrane trafficking factors are involved in the hypotonic activation of the taurine channel in the little skate (Raja erinacea) red blood cell


, : Membrane trafficking factors are involved in the hypotonic activation of the taurine channel in the little skate (Raja erinacea) red blood cell. Journal of Experimental Zoology. Part A, Comparative Experimental Biology 305(7): 594-601

In response to volume expansion, red blood cells of the little skate (Raja erinacea) initially swell and then release small organic compounds and osmotically obligated water in what is called a regulatory volume decrease (RVD) to restore cell volume. One of the major intracellular solutes lost during this process is the non-metabolized beta amino acid taurine. This hypoosmotic-induced increase in cell taurine permeability requires the anion exchanger, skAE1. The abundance of this transporter increases on the surface plasma membrane by a process of exocytosis. The second-messenger pathways involved in exocytosis of skAE1 were investigated with the use of inhibitors which affect membrane trafficking. Hypoosmotic-stimulated taurine uptake was significantly decreased by 42% with wortmannin, a phosphatidylinositol 3-kinase (P13 kinase) inhibitor. Additional evidence for the involvement of P13K was obtained with a second inhibitor, LY294002, which decreased the hypoosmotic-stimulated taurine uptake by 28%. The state of actin is also involved, as the actin filament depolymerizer latrunculin B decreased hypoosmotic-stimulated taurine uptake by approximately 40%. Although hypoosmotic conditions did not stimulate changes in the distribution of actin between filamentous and globular forms, latrunculin stimulated a decrease in filamentous actin and increase in globular actin in both isoosmotic and hypoosmotic conditions. Disruptors of other potential cytoskeletal factors (myosin, kinesin, dynein, and microtubules) did not affect taurine uptake. The present results suggest that the exocytosis of skAE1 stimulated by hyposmotic-induced cell volume expansion requires activation of P13 kinase and is regulated by the state of actin filaments.

US$19.90

PMID: 16615100

DOI: 10.1002/jez.a.292


Other references

Fehér, K.G.; Bencze, G.; Ujfalussy, J.; Fehér, T., 1987: Serum steroid hormone levels in systemic lupus erythematosus (SLE). Dehydroepiandrosterone (D), D sulphate (DS), testosterone (T) and oestradiol (OE2) were determined by radioimmunoassay; cortisol (F) by a spectrofluorimetric method in the serum of 54 female patients with SLE. The values were evaluated in two age...

Hock, B., 1967: Nature of a rapidly labeled RNA fraction described in higher plant systems. To check the effect of bacterial contamination upon the 32PO4 incorporation into nucleic acids by Citrullus vulgaris cotyledons, a bacteria free system of watermelon seedlings was obtained. During the exposure to 32PO4, a culture of Pseudomonas wa...

Ristau E.; Wittmann W., 1985: Immune response of cattle to bovine leukosis virus relevance to pathogenesis. Monatshefte fuer Veterinaermedizin 40(16): 545-548

Heck, Ludwig, 1930: Das Affenpalmenhaus des Berliner Zoologischen Gartens. [long dash]Detailed description.

Wada J.A., 1975: Comparative study of epileptic seizure development in rhesus monkey and senegalese baboon papio papio. Journal of Medical Primatology 4(6): 392

White, G.W.; Dashner, J., 2015: In Memoriam: David B. Gray, PhD, Scientist, Colleague, and Advocate. Archives of Physical Medicine and Rehabilitation 96(9): 1744-1745

Gupta U.; Gupta N.K., 1982: Nematode parasites of some hilly areas of north india. Molecular & Biochemical Parasitology (SUPPL): 335

Goldstein M.; Tiffany P., 1985: Microsurgical vasovasostomy refinements and results. Journal of Urology 133(4 PART 2): 259A

Guo, M.; Chen, J.; Yun, X.; Chen, K.; Nie, L.; Yao, S., 2006: Monitoring of cell growth and assessment of cytotoxicity using electrochemical impedance spectroscopy. Electrochemical impedance spectroscopy (EIS) was used to monitor the growth of mammalian cancer cells and evaluate the cytotoxicity of chemicals using Fe(CN63-/4- as a redox probe. Cancer cells, the human hepatocarcinoma cell line (BEL7404), were...

Capasso Barbato L.; Peronio C., 1983: Systematic and phylogenetic considerations on hippopotamus pentlandi mammalia. H. pentlandi from the grotto of Cannita (Villabate, Palermo) was examined. The systematic position of this form of hippopotamus is discussed evaluating significant morphological differences between it and H. amphibius Linnaeus from which it is der...