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Mini chamber system for long-term maintenance and observation of cultured cells

, : Mini chamber system for long-term maintenance and observation of cultured cells. Biotechniques 38(2): 267-273

We constructed a mini chamber system that was able to maintain cell culture on a microscope for long periods. It is a modified closed system with medium perfusion and CO2 circulation. The closed CO circulation and ample air inside the chamber distinguish it from other closed systems. Using different cell lines, the system was shown to be able to support long-term, time-lapse recording.


PMID: 15727133

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