geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Neuropeptide Y expression, localization and cellular transducing effects in HUVEC


, : Neuropeptide Y expression, localization and cellular transducing effects in HUVEC. Biology of the Cell 97(6): 457-467

Background information. NPY (neuropeptide Y) may have an effect on the properties of vascular endothelial cells such as pro-angiogenic effects and potentiation of noradrenaline-induced vasoconstriction. In HUVEC (human umbilical-vein endothelial cells), immunoreactive neuropeptide Y has been detected, but NPY synthesis, storage and secretion have not been studied. The aim of the present study was to establish NPY expression, storage and cellular transducing effects in HUVEC.Results. HUVEC contain 0.19 fmol of NPY/mu g of protein and 0.46 fmol of pro-NPY/mu g of protein, as measured by ELISA. RT (reverse transcriptase)-PCR confirmed the expression of NPY in HUVEC. Immunofluorescence revealed the presence of NPY in small punctate structures, with a fluorescence pattern different from that observed for von Willebrand factor, indicating distinct storage compartments. Double labelling for NPY and Rab3A demonstrated similar granular patterns, with at least partial co-localization. Electron microscopy showed NPY immunoreactivity in vesicle-like cytoplasmic structures, of a fine fibrillar texture, as well as in mitochondria and in the nucleus. A similar general distribution pattern was also obtained for Rab3A. Y1 and Y2 receptors were expressed in HUVEC as assessed by RT-PCR, and they were functional since NPY induced a 42 nM intracellular calcium increase within 100 s, representing 22% of the histamine-induced response. In contrast with histamine, NPY did not induce acute von Willebrand factor secretion.Conclusions. HUVEC produce, store and respond to NPY, suggesting an autocrine regulatory role for NPY in the endothelium.

US$19.90

PMID: 15850450

DOI: 10.1042/BC20040102


Other references

Kantor, G.L., 1970: Toxic epidermal necrolysis, azotemia, and death after allopurinol therapy. JAMA 212(3): 478-479

Sargent, D.; Taylor, C., 1972: Light-induced inhibition of respiration of DCMU-poisoned Chlorella caused by photosystem I activity. Canadian journal of botany: 50 (1) 13-21

Dowling, J., 1979: Smallpox and literature in eighteenth-century Spain. Studies in Eighteenth-Century Culture 9: 59-77

Tapper-Jones, L.; Aldred, M.; Walker, D.M., 1980: Prevalence and intraoral distribution of Candida albicans in Sjögren's syndrome. An imprint culture technique has been employed to study the prevalence and intraoral distribution of Candida albicans in 16 patients with Sjögren's syndrome and in 16 healthy controls matched for age, sex, and dental status. The prevalence a...

Pytel, A., 1960: Renal fornical hemorrhages: their pathogenesis and treatment. Journal of Urology 83: 783-789

Pelissari, C.; Guivernau, M.; Viñas, M.; de Souza, S.Silva.; García, J.; Sezerino, P.Heleno.; Ávila, C., 2017: Unraveling the active microbial populations involved in nitrogen utilization in a vertical subsurface flow constructed wetland treating urban wastewater. The dynamics of the active microbial populations involved in nitrogen transformation in a vertical subsurface flow constructed wetland (VF) treating urban wastewater was assessed. The wetland (1.5m<sup>2</sup>) operated under average l...

Park JongHee; Keon DaeKun; D.W.nIm, 2006: Pharmacognostical studies on the Jeob Gol Mog. The Korean folk medicine 'Jeob Gol Mog' has been used to cure nephritis, hypoacidity rheumatism, gastrectasis and bone fracture. With regard to the botanical origin of 'Jeob Gol Mog', it has been considered to be Sambucus speci...

Schowalter, J.E.; Friedman, C.P.; Scheiber, S.C.; Juul, D., 2003: An experiment in graduate medical education: combined residency training in pediatrics, psychiatry, and child and adolescent psychiatry. As an experiment in graduate medical education, a 5-year curriculum in pediatrics, psychiatry, and child and adolescent psychiatry was developed and evaluated over a 10-year period. The evaluation results suggested that the program's goal to...

Burris T.E.; Fulcher S.F.A.; North R.B.; Gavin M., 1986: Identification of confounding variables in corneal alkali burn modeling. Investigative Ophthalmology & Visual Science 27(3 SUPPL): 207

Puchkova, L.V., 1969: Periphyton of the water scorpion (Nepa cinerea L.). Gidrobiologicheskii Zhurnal, 5(6): 95-96