geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Peripheral protein adsorption to lipid-water interfaces: the free area theory


, : Peripheral protein adsorption to lipid-water interfaces: the free area theory. Biophysical Journal 89(6): 3997-4005

In fluid monolayers approaching collapse, phospholipids and their complexes with diacylglycerols hinder adsorption to the monolayer of the amphipathic protein, colipase. Herein, a statistical, free-area model, analogous to that used to analyze two-dimensional lipid diffusion, is developed to describe regulation by lipids of the initial rate of protein adsorption from the bulk aqueous phase to the lipid-water interface. It is successfully applied to rate data for colipase adsorption to phospholipid alone and yields realistic values of the two model parameters; the phospholipid excluded area and the critical free surface area required to initiate adsorption. The model is further developed and applied to analyze colipase adsorption rates to mixed monolayers of phospholipid and phospholipid-diacylglycerol complexes. The results are consistent with complexes being stably associated over the physiologically relevant range of lipid packing densities and being randomly distributed with uncomplexed phospholipid molecules. Thus, complexes should form in fluid regions of cellular membranes at sites of diacylglycerol generation. If so, by analogy with the behavior of colipase, increasing diacylglycerol may not trigger translocation of some amphipathic peripheral proteins until its abundance locally exceeds its mole fraction in complexes with membrane phospholipids.

US$19.90

PMID: 16150972

DOI: 10.1529/biophysj.105.061952


Other references

St-Pierre, A.C.; Bergeron, J.; Pirro, M.; Cantin, B.; Dagenais, G.R.; Després, J-Pierre.; Lamarche, Bît., 2003: Effect of plasma C-reactive protein levels in modulating the risk of coronary heart disease associated with small, dense, low-density lipoproteins in men (The Quebec Cardiovascular Study). This purpose of this study was to investigate how plasma C-reactive protein (CRP), a nonspecific acute-phase reactant, modulates the risk of coronary heart disease (CHD) associated with the small, dense, low-density lipoprotein (LDL) phenotype. LD...

Thomas, J.-A.; Leclerc, J., 1962: The prevention of foot and mouth disease in cows by the intravenous injection of purified gamma -globulin antibodies.. Cows were (i) inoculated with live foot-and-mouth virus type C, (ii) injected with gamma -globulins prepared from immune milk containing antibodies to foot-and-mouth virus (see D.S.A. 23 & 24), followed by inoculation with the live virus, or (iii)...

Weir, W.C.; Jones, L.G.; Meyer, J.H., 1960: Effect of cutting interval and stage of maturity on the digestibility and yield of alfalfa. Chemical composition, digestibility by sheep, and yield of digestible protein and total digestible nutrients per acre were obtained by a series of studies on irrigated alfalfa. In the first study, alfalfa was cut at 21, 28, 35 and 42-day intervals...

Poloméni, A.; Lapusan, S.; Bompoint, C.; Rubio, M.T.; Mohty, M., 2015: The impact of allogeneic-hematopoietic stem cell transplantation on patients' and close relatives' quality of life and relationships. Although evidence suggests considerable disruption to families, the impact of allo-Hematopoietic Stem Cell Transplantation (HSCT) on patients' partners and close relatives has not been sufficiently explored. The present mixed-methods study ai...

Benatti, M.R.; Penning, B.W.; Carpita, N.C.; McCann, M.C., 2012: We are good to grow: dynamic integration of cell wall architecture with the machinery of growth. Despite differences in cell wall composition between the type I cell walls of dicots and most monocots and the type II walls of commelinid monocots, all flowering plants respond to the same classes of growth regulators in the same tissue-specific...

Zhang, H.; Zhang, S-Bo.; Zhang, Q-Qing.; Liu, M.; He, X-Ying.; Zou, Z.; Sun, H-Jing.; You, Z-Dong.; Shi, X-Yin., 2014: Rescue of cAMP response element-binding protein signaling reversed spatial memory retention impairments induced by subanesthetic dose of propofol. Cns Neuroscience & Therapeutics 19(7): 484-493

Lyons J., 1989: Changes in the abundance of small littoral zone fishes in lake mendota wisconsin usa. Since 1900, major changes have occurred in the assemblage of small littoral-zone fishes (maximum total length < 150 mm; usually encountered in the nearshore area) that inhabits Lake Mendota, Wisconsin. A diverse assemblage that included several...

Mul'tanovskii, M.P., 1952: Seminars in medical history in medical institutes. Sovetskoe Zdravookhranenie 11(4): 15-19

Udra, If, 1984: Cornus mas (Cornaceae) in the Ukraine, the relict of the Tertiary forests. Botanicheskii zhurnal 69(1): 33-42

Rubel, C.A.; Wu, S-Pin.; Lin, L.; Wang, T.; Lanz, R.B.; Li, X.; Kommagani, R.; Franco, H.L.; Camper, S.A.; Tong, Q.; Jeong, J-Wook.; Lydon, J.P.; DeMayo, F.J., 2016: A Gata2-Dependent Transcription Network Regulates Uterine Progesterone Responsiveness and Endometrial Function. NlmCategory="UNASSIGNED">Altered progesterone responsiveness leads to female infertility and cancer, but underlying mechanisms remain unclear. Mice with uterine-specific ablation of GATA binding protein 2 (Gata2) are infertile, showin...