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Physical and functional interaction of DNA methyltransferase 3A with Mbd3 and Brg1 in mouse lymphosarcoma cells


, : Physical and functional interaction of DNA methyltransferase 3A with Mbd3 and Brg1 in mouse lymphosarcoma cells. Cancer Research 65(23): 10891-10900

Dnmt3a and Dnmt3b are de novo DNA methyltransferases that also act as transcriptional repressors independent of methyltransferase activity. To elucidate the underlying mechanism of transcriptional repression, Dnmt3a was purified from mouse lymphosarcoma cells (P1798) by extensive fractionation on five different chromatographic matrices followed by glycerol density gradient centrifugation. Liquid chromatography electrospray tandem mass spectrometry analysis of Dnmt3a-associated polypeptides identified the methyl CpG binding protein Mbd3, histone deacetylase 1(Hdac1), and components of Brg1 complex (Brg1, Baf155, and Baf57) in the purified preparation. Association of Dnmt3a with Mbd3 and Brg1 was confirmed by coinummoprecipitation and coimmunolocalization studies. Glutathione S-transferase pulldown assay showed that the NH2-terminal ATRX homology domain of Dnmt3a interacts with the methyl CpG binding domain of Mbd3 and with both bromo and ATPase domains of Brg1. Chromatin immunoprecipitation assay revealed that all three proteins are associated with transcriptionally silent methylated metallothionein (MT-I) promoter in the mouse lymphosarcoma cells. To understand the functional significance of their association with the promoter, their role on the MT-I promoter activity was analyzed by transient transfection assay. The results showed that Mbd3 and Dnmt3a specifically inhibited the methylated promoter, and the catalytic activity of Dnmt3a was dispensable for the suppression. In contrast, the wild-type but not the ATPase-inactive mutant of Brg1 suppressed MT-I promoter irrespective of its methylation status, implicating involvement of ATP-dependent chromatin remodeling in the process. Coexpression of two of the three interacting proteins at a time augmented their repressor function. This study shows physical and functional interaction of Dnmt3a with components of nucleosome remodeling machinery.

US$19.90

PMID: 16322236

DOI: 10.1158/0008-5472.CAN-05-1455


Other references

Brown, De, 1978: Grazing, grassland cover and gamebirds. Transactions 3d) 477-485

Burns, J.; Pressey, R., 1987: Ca2+ in cell walls of ripening tomato and peach. Journal of the American Society for Horticultural Science 112(5): 783-787

Tully, J.G.; Razin, S., 1970: Acholeplasma axanthum, sp. n.: a new sterol-nonrequiring member of the Mycoplasmatales. Mycoplasmas recovered from tissue cultures and previously shown to belong to the sterol-nonrequiring group of mycoplasmas have been further characterized. The biological and serological properties of these strains show them to be clearly distinct...

Swan, H.J., 1975: Balloon flotation catheters. Their use in hemodynamic monitoring in clinical practice. JAMA 233(8): 865-867

Zanardo, V.; Giarrizzo, D.; Maiolo, L.; Straface, G., 2016: Efficacy of Topical Application of Emu Oil on Areola Skin Barrier in Breastfeeding Women. Appropriate hydration and skin surface pH are of fundamental importance in preventing areola skin barrier damage and breastfeeding success. We studied the dermal effects of emu oil on areola skin soon after birth in 70 at-term breastfeeding mother...

Hutchinson, R.; Margulis, V., 2016: Editorial Comment to Spherical cap surface model: A novel method for predicting renal function after partial nephrectomy. International Journal of Urology 23(8): 673-673

Dalal, R.C., 1975: Urease activity in some Trinidad soils. Urease activity in fifteen soils was studied in relation to soil moisture, clay content, organic C, CEC, amorphous Fe and Al, and experimental conditions. In the presence of toluene, activity was mainly associated with the clay-organic matter comp...

Baxter, R.A., 2011: Update on the split-muscle technique for breast augmentation: prevention and correction of animation distortion and double-bubble deformity. Aesthetic Plastic Surgery 35(3): 426-429

Rohmann, U., 1984: Possibilities of preparation of nitrate loaded ground waters. DVGW Schriftenreihe: Wasser: 8) 168-182

Marty R., 1969: Detection of carotid artery obstruction by intra venous radio isotope arteriography. International Congress Series: