+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Preparation and characterization of nitrilotriacetic-acid-terminated self-assembled monolayers on gold surfaces for matrix-assisted laser desorption ionization-time of flight-mass spectrometry analysis of proteins and peptides

, : Preparation and characterization of nitrilotriacetic-acid-terminated self-assembled monolayers on gold surfaces for matrix-assisted laser desorption ionization-time of flight-mass spectrometry analysis of proteins and peptides. Analytical Biochemistry 345(2): 258-269

On-target affinity capture, enrichment and purification of biomolecules improve detection of specific analytes from complex biological samples in matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) analysis. In this paper, we report a simple method for preparation of a self-assembled nitrilotriacetic acid (NTA) monolayer on gold surface which can be used as a MALDI-TOF-MS sample target specifically for recombinant oligohistidine-tagged proteins/peptides and phosphorylated peptides. The NTA functional groups are immobilized to the gold surface via the linkage of 1,8-octanedithiol which forms a self-assembled monolayer on gold. Characterization by X-ray photoelectron spectroscopy and MALDI analysis of the modified surface are described. The chemically modified surface shows strong affinity toward the analytes of interest, which allows effective removal of the common interferences, e.g. salts and detergents, and therefore leads to improved signal/noise ratio and detection limit. The use of the modified surface simplifies the sample preparation for MALDI analysis of these targeted analytes. (C) 2005 Elsevier Inc. All rights reserved.


PMID: 16125121

DOI: 10.1016/j.ab.2005.07.009

Other references

Sugai Y.; Sugai K.; Hirata T.; Okuda C.; Miyazaki M., 1987: The interaction of pancuronium and vecuronium with cardiac muscarinic receptors. The interaction of vecuronium, a monoquaternary analogue of pancuronium, with the cardiac muscarinic receptors in canine hearts was investigated in vitro by [3H] QNB binding assay and compared with that of pancuronium. Both pancuronium and vecuron...

Van Os, J.; Fananas, L.; Cannon, M.; Murray, R., 1996: A study of monozygotic twins discordant and concordant for psychotic illness examining dermatoglyphic risk factors for psychosis. Schizophrenia Research 18(2-3): 172

Rapp, R.P.; Irifune, T.; Shimizu, N.; Nishiyama, N.; Norman, M.D.; Inoue, T., 2008: Subduction recycling of continental sediments and the origin of geochemically enriched reservoirs in the deep mantle. Isotopic and trace element geochemical studies of ocean island basalts (OIBs) have for many years been used to infer the presence of long-lived ( approximately 1-2 Ga old) compositional heterogeneities in the deep mantle related to recycling of cr...

Souza,; Rocha, S.Fabrício.Blattes.da.; Cordellini, M.Ferreira.; Vosgerau, R.Pereira.; Kowacs, P.André., 2013: Primary progressive apraxia: a syndrome difficult to categorize. Arquivos de Neuro-Psiquiatria 71(4): 267-268

Jacquinot, L., 1962: The elimination of hydroxides after treatment with nitric acid in the determination of total bases. Precipitation in presence of benzoate. Method used in the agropedological laboratory of the CRA in Bambey.. The HNO3 extract is evaporated to about 50 ml, brought to pH 3-4 with NH4OH, treated with 3 ml CH3COOH & dropwise with 20 ml of 10% ammonium benzoate. The solution, brought to 125 ml, is filtered; 100 ml of the filtrate is evaporated to dryness, t...

Berezetskaya, N.M.; Kharkyanen, V.N.; Kononenko, N.I., 1996: Mathematical model of pacemaker activity in bursting neurons of snail, Helix pomatia. On the basis of experimental data we have developed a mathematical model of pacemaker activity in bursting neurons of snail Helix pomatia which includes a minimal model of membrane potential oscillation, spike-generating mechanism, voltage- and ti...

Gulyani, S.; Mallick, B.N., 1995: Possible mechanism of rapid eye movement sleep deprivation induced increase in Na-K ATPase activity. Rapid eye movement sleep deprivation increases Na-K ATPase activity and decreases aminergic neuronal firing rate as well as norepinephrine degrading enzyme, monoamine oxidase, activity. On the other hand, norepinephrine is known to increase Na-K A...

Ingarfill, E.W., 1954: A foot-operated stop watch. Journal of Medical Laboratory Technology 12(1): 40-42

Sedova, Z.A.; Osipova, Z.F., 1975: Chemical and technological appraisal of some raspberry and strawberry varieties. Data on fruit quality, assessed in eleven raspberry and seven strawberry varieties between 1968 and 1973, are tabulated. Among the raspberries, Progress, Garnet and Novost' Kuz'mina [Kuz'min's Novelty] had the highest dry-matte...

Mozer, T.J.; Thompson, R.B.; Berget, S.M.; Warner, H.R., 1977: Isolation and characterization of a bacteriophage T5 mutant deficient in deoxynucleoside 5'-monophosphatase activity. A bacteriophage T5 mutant has been isolated that is completely deficient in the induction of deoxynucleoside 5'-monophosphatase activity during infection of Escherichia coli F. The mutant bacteriophage has been shown to be deficient in the ex...