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Production of cyclofructan by cycloinulooligosaccharide fructanotransferase expressed in Saccharomyces cerevisiae

, : Production of cyclofructan by cycloinulooligosaccharide fructanotransferase expressed in Saccharomyces cerevisiae. Korean Journal of Microbiology & Biotechnology 32(1): 60-66, March

The cycloinulooligosaccharide fructanotransferase (CFTase) gene (cft) from Paenibacillus polymyxa was subcloned into the E. coli-yeast shuttle vector, pYES2.0 (GAL1 promoter). The constructed plasmid, pYGCFT (9.9 kb) was introduced into S. cerevisiae SEY2102 cell and then the yeast transformant was selected on the synthetic defined media lacking uracil. Based on the cyclofructan (CF) spots on thin-layer chromatogram, the cft gene under the control of GAL I promoter was successfully expressed in the yeast transformant. The recombinant CFTase was not secreted into the medium and was predominantly localized in the periplasmic space. CF was started to be produced after 3 h of enzymatic reaction with inulin. The pH and temperature optimum for the CF production from inulin was pH 8.0 and 45degreeC, respectively. Enzyme activity was stably maintained up to the pH of 10.0. The examination of the inulin sources revealed that a dahlia tuber and Jerusalem artichoke were the best for the production of CF.


Other references

Lukola A.; Lovgren T.; Punnonen R., 1980: Nuclear binding of estradiol in human myometrium. The nuclear uptake of human myometrial estrogen receptors in cytosol prepared in a low salt medium in the presence and absence of diisopropylfluorophosphate (DFP) was studied. The [3H]-estradiol receptor complex in the cytosol prepared in the pres...

Marchisio, P.; Selicorni, A.; Bianchini, S.; Milani, D.; Baggi, E.; Cerutti, M.; Larizza, L.; Principi, N.; Esposito, S., 2015: Audiological findings, genotype and clinical severity score in Cornelia de Lange syndrome. Cornelia de Lange syndrome (CdLS) is a rare multisystem disorder in which hearing loss (HL) has been reported. However, no data are available concerning the association between audiological findings, clinical severity score and genotype. The study...

Opresko, L.K.; Chang, C.P.; Will, B.H.; Burke, P.M.; Gill, G.N.; Wiley, H.S., 1993: Removal of the tyrosine kinase domain leads to constitutive internalization and lysosomal targeting of the EGF receptor. Molecular Biology of the Cell 4(SUPPL ): 433A

Ramos Monreal, A., 2002: Grain legume genetic resources in Spain. The status of the national grain legume collections in Spain, containing 13 400 accessions, including 7805 accessions of Spanish origin and 6543 landraces, are described. The collecting activities; conservation, utilization and documentation of th...

Sukenik, S.; Hendler, N.; Yerushalmi, B.; Buskila, D.; Liberman, N., 1991: Jaccoud's-type arthropathy: an association with sarcoidosis. We describe a patient with sarcoidosis involving the lungs, muscles and tendons of the forearms who developed Jaccoud's-type arthropathy of the hands. This is the first reported case to the best of our knowledge of sarcoidosis associated with...

Gulyaeva, EP., 1972: Ecologo-physiological adaptation of heart-vascular system in minks (Mustela vison Brisson) to diving and its change as a result of their domestication. Teriologiya, 1: 303-311

Sun, J-Jun.; Yang, T-Bao.; Yang, Y-Hui.; Liu, W-Feng.; Song, J-Xin., 2016: Synchronous double primary malignancies of the liver and kidney: A case report. NlmCategory="UNASSIGNED">A 42-year-old male patient was admitted to the Department of General Surgery of The First Affiliated Hospital of Henan University of Science and Technology (Luoyang, China) presenting with abdominal discomfort...

Curnow, A.W.; Garcia, G.A., 1994: Investigations into the interaction of E coli tRNA-tyr analogues and the post-transcriptional hypermodification enzyme tRNA-guanine transglycosylase. FASEB Journal 8(7): A1348

Uribarri, J.; Bottone, E.J.; London, R.D., 1996: Pasteurella multocida peritonitis: are peritoneal dialysis patients on cyclers at increased risk?. Peritoneal Dialysis International 16(6): 648-649

Hussey, Keith M., 1949: Louisiana Cane River Eocene Foraminifera. The study brought out a close correlation, both lithological and paleontological, between the Cane River formation of Louisiana, the Weches of Texas and the upper Tallahatta of Mississippi. The foraminiferal fauna descr. comprises well over 100 sp...