+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Productive replication of hepatitis C virus in perihepatic lymph nodes in vivo: implications of HCV lymphotropism

, : Productive replication of hepatitis C virus in perihepatic lymph nodes in vivo: implications of HCV lymphotropism. Gastroenterology 130(4): 1107-1116

Background & Aims: The pathogenesis of chronic hepatitis C is poorly understood. This study examines the ability of hepatitis C virus (HCV) to infect, replicate in, and produce progeny virus from perihepatic lymph nodes in vivo. Methods: Lymph node (LN) biopsy specimens were taken from 20 patients with HCV genotype 1. infection and end-stage liver disease and 20 noninfected negative controls. Sections were probed with HCV RNA strand-specific riboprobes and antibodies specific for HCV core and nonstructural region 3 antigens plus B-cell (CD20) and T-cell (CD2) antigens. In a selected case, HCV quasispecies in serum, peripheral blood mononuclear cells, liver, and perihepatic lymph nodes were analyzed by clonal frequency analysis and sequencing. Results: HCV infection was confirmed in 17 of 20 (85%) of lymph node specimens by in situ hybridization, and HCV replication was confirmed in 50% of cases by detection of HCV replicative intermediate RNA. HCV core and nonstructural 3 antigens were detected in lymph nodes by immunocytochemistry. Infected cell phenotypes were primarily CD20 B cells, although other cell types were positive for HCV replication markers. Quasispecies analysis in one case indicated that 68% of variants circulating in serum were also present in lymphoid tissues, and only 40% of serum variants were identified in liver, documenting a major contribution of lymphoid replication to HCV viremia. Conclusions: HCV lymphotropism provides new insights into the complex pathobiology of chronic hepatitis C in humans. We demonstrate for the first time a major contribution of extrahepatic HCV replication to circulating virus in serum (viremia).


PMID: 16618405

DOI: 10.1053/j.gastro.2005.12.039

Other references

Winker Gregory J.; Berg Robert R.; Tieh Thomas T., 1983: Facies and diagenetic controls on reservoir rock properties of Hosston sandstones, Washington Parish, Louisiana. AAPG Bulletin 67(3): 570-571

Barsotti, G., 1973: Shells of all seas: bivalves. Conchiglia, 52: 11-14

Hu, G.; Heitmann, J.A.; Rojas, O.J., 2010: In situ monitoring of cellulase activity by microgravimetry with a quartz crystal microbalance. Quartz crystal microgravimetry (QCM) was used to investigate the interactions between cellulase enzymes and model cellulose substrates. The substrates consisted of thin films of cellulose that were spin-coated onto polyvinylamine (PVAm) precoated...

Klotzsche, O.; Eismann, T.; Bohn, W.; Deppert, W., 1999: Vimentin dependant cytoplasmic anchorage of tsp53 has functional relevance for its wildtype activities at permissive temperature. European Journal of Cell Biology 78(SUPPL 49): 24

Anikin, B.S.; Kaganov, A.L.; Tarlova, R.M., 1976: Evaluation of polychemotherapy and its complications in late stages of Hodgkin's disease. Terapevticheskii Arkhiv 48(8): 73-79

Lazurkin V.M., 1957: Geological setting of lower reaches of Lena River region, Lena Basin. Trudy - Nauchno-Issledovatel'skiy Institut Geologii Arktiki 81: 461-483

Stevenson, D.E., 1969: The saftey evaluation of pet products. Veterinary Record 84(7): 168-170

Dewey, D., 2014: Cochrane Review Brief: Nurse Versus Physician-Led Care for the Management of Asthma. Online Journal of Issues in Nursing 19(3): 11-11

Samy, E.T.; Cheng, C.Y., 1998: Characterization of the 5-flanking region of the rat testin gene. Molecular Biology of the Cell 9(SUPPL ): 72A, Nov

Overdieck, D., 1985: CO2 gas exchange of the understory plant Asarum europaeum L. in November. International journal of biometeorology 29(1): 57-65