geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Somatostatin receptors in primary human breast cancer: quantitative analysis of mRNA for subtypes 1--5 and correlation with receptor protein expression and tumor pathology


, : Somatostatin receptors in primary human breast cancer: quantitative analysis of mRNA for subtypes 1--5 and correlation with receptor protein expression and tumor pathology. Breast Cancer Research and Treatment 92(2): 175-186

Somatostatin receptors (SSTRs) have been identified in most hormone-producing tumors as well as in breast cancer. In the present study, we determined SSTR1-5 expression in primary ductal NOS breast tumors through semi-quantitative RT-PCR and immunocytochemistry. The results from the analysis of 98 samples were correlated with several key histological markers and receptor expression. All five SSTR subtypes are variably expressed at the mRNA level in breast tumors with 91% of samples showing SSTR1, 98% SSTR2, 96% SSTR3, 76% SSTR4, and 54% SSTR5. SSTR1-5 are localized to both tumor cells and the surrounding peritumoral regions as detected by immunocytochemistry. Levels of SSTR mRNA, when corrected for beta-actin levels, were highest for SSTR3 followed by SSTR1, SSTR2, SSTR5, and SSTR4. Furthermore, there was good correlation between mRNA and protein expression with 84% for SSTR1, 79% for SSTR2, 89% for SSTR3, 68% for SSTR4, 68% for SSTR5, and 78% for all five receptors. SSTR1, 2 and 4 were correlated with ER levels whereas SSTR2 showed an additional correlation with PR levels. These correlations were independent of patient age and histological grade. Moreover, using immunocytochemistry, blood vessels exhibited receptor-specific localization for SSTR2 and SSTR5. Our results indicate significant correlations between mRNA and protein expression along with receptor-specific correlations with histological markers as well as ER and PR levels. Differential distribution of SSTR subtypes in tumors and receptor-specific expression in vascular structures may be considered as a novel diagnosis for breast tumors with receptor subtype agonists.

US$19.90

PMID: 15986128

DOI: 10.1007/s10549-005-2414-0


Other references

Girdhar, O.P.; Garg, S.R., 2002: Prevalence of Listeria in animal farms. This study was conducted to investigate the prevalence of Listeria monocytogenes and other Listeria spp. in the environment of some animal farms. 240 samples consisting of 83 faecal matter, 45 milk, 21 soil, 21 water, 18 sewage, 21 feed, 23 fodder...

Furuya, T.; Hasegawa, A.; Miyazawa, T.; Miki, K.; Mikami, T., 1992: Detection of anti-gag antibodies of feline immunodeficiency virus in cat sera by enzyme-linked immunosorbent assay. Using gag protein of feline immunodeficiency virus (FIV) expressed in Escherichia coli, an enzyme-linked immunosorbent assay (ELISA) system was developed for detection of antibodies to FIV gag protein in cat sera. With serum samples from cats expe...

Castellazzo, M., 1958: Experiences with finalgon ointment as contact medium in ultrasonic therapy. Gazzetta Medica Italiana 117(8): 340-341

Rezazadeh, S.M.hdi; Prather, P.L.; Lal, H., 1993: Sensitization to 5-HT-1C receptor agonist in rats observed following withdrawal from chronic ethanol. Anxiogenic action of m-chlorophenylpiperazine (mCPP), a 5-HT-1C receptor agonists, was studied in naive rats and in ethanol-tolerant rats following withdrawal from chronic ethanol administration. The purpose of this investigation was to determine...

Byrd, M.E., 1997: A typology of the potential outcomes of maternal-child home visits: a literature analysis. Home visiting the maternal-child population has been a major focus of public health nursing. The focus of a large portion of empirical studies of maternal-child home visiting has been on the demonstration of outcomes. Despite a general consensus a...

Egan, H.E., 1948: Evaluation of the coliform index of pasteurized milk. Medical Bulletin. United States. Army. European Command. Medical Division 5(5): 15-15

Godfrey, E.; Christy, K., 1992: The value and use of wilderness lands: Are they small or large at the margin?. General technical report SE US Department of Agriculture Forest Service Southeastern Forest Experiment Station: (78) 3-15

Bohm, K.H.; Trautwein, G.; Weiland, E.; Abdallah, I.S., 1974: Experimental infection of pigeons and fowls with Cryptococcus neoformans; epidemiological studies. Pigeons (Columbia livia) and fowls were experimentally infected with well capsulated or nearly uncapsulated C. neoformans (Cr.n.) strains. In pigeons the intramuscular and intracerebral routes were successful. Excretion of Cr.n. was demonstrated i...

Stripling, J.S.; Galupo, M.Paz., 2009: Differential potentiation of early and late components evoked in olfactory cortex by stimulation of cortical association fibers. The present study examined in detail the development and decay of potentiation induced in vivo by repeated high-frequency stimulation of cortical association fibers (AF) in piriform cortex (PC). Male Long-Evans rats with chronically-implanted stim...

Schrader, K.A., 2017: The Role of Hereditary Factors in Ovarian Carcinoma. Cancer is a disease of the genome, in which mutations in particular genes and pathways give rise to tissue-specific genotype-phenotype correlations. In tumors associated with hereditary cancer syndromes, a person is generally born with the first m...