geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Suppression of adenoviral gene expression in the liver: role of innate vs adaptive immunity and their cell lysis mechanisms


, : Suppression of adenoviral gene expression in the liver: role of innate vs adaptive immunity and their cell lysis mechanisms. Liver International 25(3): 622-632

Background: Injection of adenoviral constructs causes liver infection prompting immunity which suppress viral gene expression. Innate, and adaptive immunity mediate these processes raising the question which pathways are the most prominent. Methods: Adenovirus expressing the beta-galactosidase (beta-gal) gene was injected into normal and immunodeficient mice. Elimination of beta-gal-expressing hepatocytes and increases in liver enzymes were assayed. Major histocompatibility complex (MHC) class I densities, perforin channel insertion and apoptosis by Fas and tumor necrosis factor (TNF)-alpha were assayed. Results: At high virus doses, suppression of viral gene expression was as efficient in immunodeficient as in normal mice, while at low doses effects of cytotoxic T lymphocytes (CTL) were demonstrable. Despite CTL priming and elimination of infected hepatocytes no liver injury is detected. Hepatocyte MHC I densities were able to trigger CTL granule exocytosis and perforin lysis in vitro but not in vivo. This is we show is because of decreased sensitivity of hepatocytes from infected mice to perforin and increased sensitivity to Fas and TNF-alpha lysis. Conclusion: Effector cells of the innate immune system are exceedingly effective in suppressing adenoviral gene expression. Perforin-independent pathways, those mediated by TNF-alpha and Fas are very efficient in hepatocytes from virus-infected livers.

US$19.90

PMID: 15910500

DOI: 10.1111/j.1478-3231.2005.01097.x


Other references

Bult, C.J.; Sweere, J.A.; Zimmer, E.A., 1995: Cryptic sequence simplicity, nucleotide composition bias, and molecular coevolution in the large subunit of ribosomal DNA in plants: Implications for phylogenetic analyses. Sequences of the large subunit (LSU) of ribosomal DNA from Arabidopsis thaliana, Brassica napus, Sinapsis alba, Oryza saliva, Fragaria times ananassa, Lycopersicon esculentum, and Citrus limon were analyzed for nucleotide composition, presence of...

O'connor, R.; Riley, K.; Epstein, J.; Wilson, R.; Mckelvie, K.; Reynolds, A.; Hagerman, R.; Levine, R., 2002: Analysis of ADHD subtypes in Fragile X syndrome. European Journal of Human Genetics 10(Supplement 1): 323

Walker, A.; Udupa, K.B.; Chowdhury, P., 2008: Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study. The aim of the current study was to investigate the oxidative effects of nicotine by examining the mitogenic and functional responses in AR42J cells. As a control and for comparison, hydrogen peroxide (H2O2) was used as a source of known oxidativ...

Bicer, S.; Reiser, P.J., 2004: Slow fibers in orbital and global layers of dog extraocular muscles express different isoforms of myosin light chain 1. Initial examination of the low molecular weight (less than 45 kDa) protein composition of canine extraocular (rectus) muscle homogenates, based on gel electrophoresis, revealed a distinct difference between global and orbital layers of the muscles...

Ang, KJ.; Gopinath, R.; Chua, TE., 1989: The status of introduced fish species in Malaysia. Asian Fisheries Society Special Publication, 3: 71-82

Lawrence, J.R.; Shepherd, J.T.; Bone, I.; Rogen, A.S.; Fulton, W.F.M., 1980: Fibrinolytic therapy in unstable angina pectoris a controlled clinical trial. Patients [40] with persistently unstable angina pectoris were randomly allocated to treatment with streptokinase infusion for 24 h followed by oral anticoagulation with warfarin or to warfarin therapy alone. Conclusions from so small a series must...

Taira, T.; Marcussen, N.; Nikoukar, A.R.hbar; Monckton, E.; Solez, K., 1992: Immunohistochemical localization of epidermal growth factor in rabbits with ischemic acute renal failure. Journal of the American Society of Nephrology 3(3): 715

Carl, E.; Demeshko, S.; Meyer, F.; Stalke, D., 2015: Triimidosulfonates as Acute Bite-Angle Chelates: Slow Relaxation of the Magnetization in Zero Field and Hysteresis Loop of a Co(II) Complex. Starting from a polyimido sulfonate the four-coordinate, N,N'-chelated Co(II) complex [Co{(NtBu)3 SMe}2 ] (1) was synthesized, and its molecular structure was elucidated by single-crystal X-ray structural analysis. The acute N-Co-N bite angle...

Gottwald B.A., 1983: Cooperative lipid activation of sodium potassium atpase as a consequence of noncooperative lipid protein interactions. Biochimica Et Biophysica Acta: 332-335

Xia, X-yan.; Sameshima, H.; Ota, A.; Xia, Y-xin.; Ikenoue, T.; Toshimori, K.; Huang, X-hua., 2004: Effect of propentofylline on hypoxic-ischaemic brain damage in newborn rat. Background Studies showed that propentofylline enhances the action of adenosine and protects hippocampal neuronal damage against transient global cerebral ischaemia. Our study was to investigate the effect of propentofylline on hypoxic-ischaemic b...