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The human semaphorin 6B gene is down regulated by PPARs

, : The human semaphorin 6B gene is down regulated by PPARs. Genomics 83(6): 1141-1150

The peroxisome proliferator-activated receptors (PPARs) are ligand-inducible transcription factors and belong to the nuclear hormone receptor superfamily. They form heterodimers with the retinoid X receptor and bind to specific peroxisome proliferator-response elements. The latter are direct repeat elements of two hexanucleotides with the consensus sequence TG(A/T)CCT separated by a single nucleotide spacer. Such a sequence, or a similar one, has been found in numerous PPAR-inducible genes. We developed an affinity method to isolate human genomic fragments containing binding sites for PPARs and to identify novel PPAR target genes. For this, an antibody raised against all PPAR subtypes was used. Immunoselected fragments were amplified and sequenced and one of them, ISF5148, was found to bind specifically to PPARs ill gel mobility shift, supershift, and competition assays and to exhibit a down transregulation potentiality in transfection experiments under clofibrate (a PPARalpha agonist) treatment. ISF5148 was mapped by BLAST analysis 8.5 kb upstream of the human semaphorin 6B ((HSA)SEMA6B) gene. The latter encodes a member of the semaphorin family of axon guidance molecules. Expression Of this gene in human glioblastoma T98G cells was strongly down regulated after treatment with clofibrate or Wy-14,643, two PPARalpha agonists. Our study establishes for the first time that PPAR activators diminish the expression of the human (HSA)SEMA6B gene. These data are relevant to the fact that PPARs are implicated in brain development, neuronal differentiation, and lipid metabolism in the central nervous system. In addition, cross talk between the peroxisome proliferator and retinoic acid pathways is suggested.


PMID: 15177567

DOI: 10.1016/j.ygeno.2004.01.002

Other references

Olivier, S.B., 1952: Contribucion al conocimiento limnologico de la Laguna Salada Grande (Provincia de Buenos Aires, Argentina). I. Distribucion horizontal del plancton. Revista Brasileira de Biologia, 122: 161-180

Kinoshita, S.; Kanai, H.; Ikushima, T.; Sekiyama, E.; Yoneda, K., 2005: Age-related alteration of visual function and its prevention. Nihon Ronen Igakkai Zasshi. Japanese Journal of Geriatrics 41(6): 604-606

Khan, M.Altaf.; Sadaf, S.; Sajjad, M.; Waheed Akhtar, M., 2009: Production enhancement and refolding of caprine growth hormone expressed in Escherichia coli. This study describes comparison between IPTG and lactose induction on expression of caprine growth hormone (cGH), enhancing cell densities of Escherichia coli cultures and refolding the recombinant cGH, produced as inclusion bodies, to biologicall...

Li, X.; Yang, Q.; Hu, Y., 2005: Gene expression profiling reveals the mechanism of action of anticonvulsant drug QYS. We have examined the gene expression profiling of inferior colliculus from DBA/2J mice with high-intensity noise induced audiogenic seizure (AGS). We have also tested the effects of Qingyangshenylycosides (QYS), a traditional Chinese medicine, on...

Colaco, P.J.; Santos, P.J., 1999: Is the two speed test an useful tool to evaluate the anaerobic capacity of an 800m runner?. Medicine & Science in Sports & Exercise 31(5 SUPPL ): S257, May

Graf, H., 1953: Experiences with gantrisin in diseases of the throat-nose-ear region. Die Medizinische 21(6): 193-194

Anonymous., 1980: Marine observer's log. Insect. Marine Observer, 50: 21-22 No. 267

Schutte, L.; Meertens, R.M.; Mevissen, F.E.F.; Schaalma, H.; Meijer, S.; Kok, G., 2016: Long Live Love. The implementation of a school-based sex-education program in The Netherlands. Implementation of health education programs is often inadequately considered or not considered at all in planning, developing and evaluating interventions. With the focus being predominantly on the adoption stage, little is known about the factors...

Ali, A.M.M.; Khan, M.R., 1994: A comprehensive bibliography on agriculture and rural development. This is the first comprehensive bibliography to be published on agriculture and rural development in Bangladesh. It contains 7546 entries and author and subject indexes. The entries are divided into eleven chapters: agrarian reform and rural devel...

Fawcett, J., 1980: Lithium carbonate in medicine and psychiatry. Advances in Experimental Medicine and Biology 127: 1-13