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The hydroxylation of vanillate and its conversion to methoxyhydroquinone by a strain of Pseudomonas fluorescens devoid of demethylase and methylhydroxylase activities


, : The hydroxylation of vanillate and its conversion to methoxyhydroquinone by a strain of Pseudomonas fluorescens devoid of demethylase and methylhydroxylase activities. World Journal of Microbiology and Biotechnology 20(8): 827-832

A vanillate (4-hydroxy-3-methoxybenzoate)-utilizing bacterium that is unable to utilize p-cresol(4-methylphenol) or 2,4-xylenol (2,4-dimethylphenol) as sole source of carbon and energy was isolated and identified as Pseudomonas. fluorescens. The organism employs an inducible hydroxylase (decarboxylating), a fungal mode of attack, rather than a demethylase or methylhydroxylase as the initial step in vanillate metabolism.

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DOI: 10.1007/s11274-004-0871-y


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