+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The hyperfluidization of mammalian cell membranes acts as a signal to initiate the heat shock protein response

, : The hyperfluidization of mammalian cell membranes acts as a signal to initiate the heat shock protein response. Febs Journal 272(23): 6077-6086

The concentrations of two structurally distinct membrane fluidizers, the local anesthetic benzyl alcohol (BA) and heptanol (HE), were used at concentrations so that their addition to K562 cells caused identical increases in the level of plasma membrane fluidity as tested by 1,6-diphenyl-1,3,5-hexatriene (DPH) anisotropy. The level of membrane fluidization induced by the chemical agents on isolated membranes at such concentrations corresponded to the membrane fluidity increase seen during a thermal shift up to 42 degrees C. The formation of iso fluid membrane states in response to the administration of BA or HE resulted in almost identical downshifts in the temperature thresholds of the heat shock response, accompanied by increases in the expression of genes for stress proteins such as heat shock protein (HSP)-70 at the physiological temperature. Similarly to thermal stress, the exposure of the cells to these membrane fluidizers elicited nearly identical increases of cytosolic Ca2+ concentration in both Ca2+-containing and Ca2+-free media and also closely similar extents of increase in mitochondrial hyperpolarization. We obtained no evidence that the activation of heat shock protein expression by membrane fluidizers is induced by a protein-unfolding signal. We suggest, that the increase of fluidity in specific membrane domains, together with subsequent alterations in key cellular events are converted into signal(s) leading to activation of heat shock genes.


PMID: 16302971

DOI: 10.1111/j.1742-4658.2005.04999.x

Other references

Crawford F.T.; Brock O.G., 1976: Tonic immobility in venomous north american snakes. American Zoologist 16(2): 232

Gordon, I.B.; Gorbatovskaia, N.S.; Rykov, V.A., 1976: Correlation of diffuse aspergillosis and lung cancer developing against a background of chronic lympholeukemia. Klinicheskaia Meditsina 54(8): 133-134

Chambers, R.J.; Helyer, N.L.; Sopp, P.I.; Brobyn, P.; Palmer, A.; Gill, G., 1990: Entomological research at the Institute of Horticultural Research, Littlehampton, UK. A brief outline is given of work on the control of western flower thrips [Frankliniella occidentalis]. Phytotoxicity tests of chemicals used to control F. occidentalis were carried out on greenhouse crops. Pyrazophos was the safest compound tested...

Rusoff A.C., 1990: Regeneration of centrifugal fibers to the retina in cichlid fish. Society for Neuroscience Abstracts 16(2): 1001

Luks, R.V.; Domburg, G.E.; Veveris, G.P., 1989: Thermal analysis of structural modifications of cellulose. Thermal and X-ray analysis were used to study the features of the thermal degradation of 4 cellulose preparations in the range 20 degrees -475 degrees C. The greatest changes occurred in the range 250-350 degrees C, i.e. the stage of active therma...

Korschelt, E., 1906: Ueber die Entstehungsgeschichte und weitere Entwicklung des Zoologischen Instituts der Universitat Marburg. Verhandlungen der Deutschen Zoologischen Gesellschaft Leipzig, 16 19-26

Tachibana, K.; Umezawa, A.; Kato, S.; Takano, T., 1988: Nucleotide sequence of a new YPT1-related human cDNA which belongs to the ras gene superfamily. Nucleic Acids Research 16(21): 10368-10368

Bell, D., 2006: The legal framework for end of life care: a United Kingdom perspective. Intensive Care Medicine 33(1): 158-162

Anonymous, 2013: Space-simulation study reveals sodium rhythms in the body. A new study in humans published by Cell Press on Jan. 9 in the journal Cell Metabolism reveals that sodium levels fluctuate rhythmically over the course of weeks in the body, independent of salt intake. This one-of-a-kind study, which examined cos...

Hilliard, P.; Zourikian, N.; Blanchette, V.; Chan, A.; Elliott, B.; Israels, S.J.; Nilson, J.; Poon, M-C.; Laferriere, N.; Van Neste, C.; Jarock, C.; Wu, J.; McLimont, M.; Feldman, B., 2013: Musculoskeletal health of subjects with hemophilia A treated with tailored prophylaxis: Canadian Hemophilia Primary Prophylaxis (CHPS) Study. Full-dose prophylaxis is very effective at minimizing joint damage but is costly. Tailored prophylaxis has been proposed as a way of reducing costs while still protecting joints. To report detailed findings in index joints of 56 subjects with seve...